Rat IL-4 Recombinant Rabbit Monoclonal Antibody [PSH04-21] - BSA and Azide free (Capture)
概述
产品名称
Rat IL-4 Recombinant Rabbit Monoclonal Antibody [PSH04-21] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Rat IL-4 aa 25-147 (P20096).
种属反应性
Rat
验证应用
ELISA(Cap)
分子量
Predicted band size: 16.2 kDa
阳性对照
Recombinant Rat IL-4 protein (HA210759).
偶联
unconjugated
克隆号
PSH04-21
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-22] to Rat IL-4 (Detector) (HA722105) and recombinant standard Rat IL-4 protein (HA210759). The reference range value is 8.2-2000 pg/ml.
靶点
功能
The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. IL-4 is produced primarily by mast cells, Th2 cells, eosinophils and basophils. It is closely related and has functions similar to IL-13. Interleukin 4 has many biological roles, including the stimulation of activated B cell and T cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B cell class switching to IgE, and up-regulates MHC class II production. IL-4 decreases the production of Th1 cells, macrophages, IFNγ, and dendritic cells IL-12. Overproduction of IL-4 is associated with allergies.
背景文献
1. Zhang J et al. IL4-driven microglia modulate stress resilience through BDNF-dependent neurogenesis. Sci Adv. 2021 Mar
2. Arpa L et al. Distinct Responses to IL4 in Macrophages Mediated by JNK. Cells. 2023 Apr
亚细胞定位
Secreted.
UNIPROT
别名
B cell growth factor 1 antibody
B cell IgG differentiation factor antibody
B Cell Stimulatory Factor 1 antibody
B-cell stimulatory factor 1 antibody
BCGF 1 antibody
BCGF1 antibody
Binetrakin antibody
BSF-1 antibody
BSF1 antibody
IGG1 induction factor antibody
展开图片
-
Sandwich ELISA analysis of Rat IL-4 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722104) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IL-4 protein (HA210759) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722105) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"