Human IL-23A Recombinant Rabbit Monoclonal Antibody [PSH03-03] - BSA and Azide free (Capture)
概述
产品名称
Human IL-23A Recombinant Rabbit Monoclonal Antibody [PSH03-03] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human IL-23A aa 20-189.
产品特异性
Human IL-23A (PSH03-03) Rabbit mAb detects endogenous levels of IL-23 P19.
种属反应性
Human
验证应用
ELISA(Cap)
分子量
Predicted band size: 20.7 kDa
阳性对照
Recombinant Human IL-23 protein (HA210909).
偶联
unconjugated
克隆号
PSH03-03
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-04] to Human Human IL-23 (Detector) (HA721934) and recombinant Human IL-23 protein (HA210909) as the standard. The reference range value is 37-3000 pg/ml.
靶点
功能
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
背景文献
1. Oppmann B., Lesley R., Blom B., Timans J.C., Xu Y., Hunte B., Vega F., Yu N. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 13:715-725 (2000).
2. Sun R., Abraham C. IL23 Promotes Antimicrobial Pathways in Human Macrophages, Which Are Reduced With the IBD-Protective IL23R R381Q Variant. Cell. Mol. Gastroenterol. Hepatol. 10:673-697 (2020).
亚细胞定位
Secreted.
UNIPROT
别名
IL 23
IL 23 A
IL 23 subunit alpha
IL 23A
IL 23p19
IL-23 subunit alpha
IL-23-A
IL-23p19
IL12B
IL23
展开图片
-
Sandwich ELISA analysis of human IL-23 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721933) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-23 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721934)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Conditioned media was harvested after 16 hours. IL-23 was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-23 were interpolated from the IL-23 standard curves. The mean IL-23 concentration was determined to be 1407 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"