Human IFN-alpha Recombinant Rabbit Monoclonal Antibody [PS00-80] - BSA and Azide free (Capture)
概述
产品名称
Human IFN-alpha Recombinant Rabbit Monoclonal Antibody [PS00-80] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant full length protein.
种属反应性
Human
验证应用
ELISA(Cap)
分子量
Predicted band size: 22 kDa
阳性对照
Recombinant human IFN-alpha protein.
偶联
unconjugated
克隆号
PS00-80
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS00-79] to IFN-alpha (Detector) (HA721291).
靶点
功能
IFN-alpha is a pharmaceutical drug composed of natural interferon alpha (IFN-α). Interferon alfa is used in a variety of treatments, including certain forms of leukemia, malignant melanoma, non-Hodgkin's lymphoma, hepatitis B, and hepatitis C. It is typically administered as an injection under the skin. Interferon alfa contains a mixture of several proteins, all with structural, serological, and functional properties typical for natural interferon alpha (IFN-α). IFN-α8 enhances the proliferation of human B cells, as well as being able to activate NK cells. The subtypes α10 and α2, along with α8, are the most efficient and powerful NK cell activators. Subtypes α21 and α2 enhance the expression of IFN-gamma-inducible protein-10 (IP-10) in dendritic cells. Activated dendritic cells initiate immune responses and induce the expression of IP-10, a chemokine which promotes a Th1 inflammatory response. IFN-α1 causes increased HLA-II expression, and can directly inhibit tumor cell growth in vitro. However, it is a poor activator of NK cells, has relatively little antiviral activity, does not induce B cell proliferation, and does not enhance HLA-I or tumor antigen expression. Despite its apparent inactivity, it is still used clinically in the treatment of metastatic renal cell carcinoma, with a reported lower toxicity than the recombinant IFN-α2. Overall, IFN-α has a general inflammatory action which skews the immune response towards a Th1 profile.
背景文献
1. Woo MH, Burnakis TG (March 1997). "Interferon alfa in the treatment of chronic viral hepatitis B and C". The Annals of Pharmacotherapy. 31 (3): 330–7.
2. "Interferon alfa - Drug Information - Chemocare". chemocare.com. Retrieved 2021-10-17.
亚细胞定位
Secreted.
UNIPROT
别名
IFL antibody
IFN alpha antibody
IFN alpha 1/13 antibody
IFN alpha 1b antibody
IFN alphaD antibody
IFN antibody
IFN-alpha-1/13 antibody
IFNA@ antibody
IFNA1 antibody
IFNA1_HUMAN antibody
展开图片
-
The binding activity of IFN-alpha (HA721290) with recombinant human IFN-alpha protein.
Immobilized recombinant human IFN-alpha protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721290) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Standard curve of IFN-alpha matched pair antibodies:<br /><br />Sandwich ELISA analysis of IFN-alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA721290" style="font-weight: bold;text-decoration: underline;">HA721290</a> [PS00-80] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IFN-alpha protein starting from 20 ug/ml to 19 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-79] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721290_2.jpg?v=20240904184735)
Standard curve of IFN-alpha matched pair antibodies:
Sandwich ELISA analysis of IFN-alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721290 [PS00-80] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IFN-alpha protein starting from 20 ug/ml to 19 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-79] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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