PCSK9 Mouse Monoclonal Antibody [2F1]
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Catalog# EM1701-26
PCSK9 Mouse Monoclonal Antibody [2F1]
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
概述
产品名称
PCSK9 Mouse Monoclonal Antibody [2F1]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein with Human PCSK9 1-201 / 692.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IF-Cell, FC
分子量
Predicted band size: 74 kDa
阳性对照
PANC-1, H22, SW1990, HepG2, human liver tissue, human colon cancer tissue, human kidney tissue, mouse kidney tissue.
偶联
unconjugated
克隆号
2F1
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:200
靶点
功能
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC-1, is a 692 amino acid protein that belongs to the peptidase S8 family and contains one peptidase S8 domain. Important in the regulation of plasma cholesterol homeostasis, PCSK9 binds to low-density lipid receptor family members LDLR, very low-density lipid receptor (VLDLR) and apolipoprotein receptor 2 (ApoER2) and promotes their degradation in intracellular acidic compartments. PCSK9 also plays a role in neuronal differentiation and apoptosis. PCSK9 is expressed in Shwann cells, neuro-epithelioma, colon carcinoma, and hepatic and pancreatic cell lines. PCSK9 levels in the brain are highest in the cerebellum during perinatal development, with ischemia causing increased levels in the adult brain. Defects in the gene encoding this protein causes the autosomal dominant disorder familial hypercholesterolemia 3 (HCHOLA3).
背景文献
1. Lai Q et al. E2F1 inhibits circulating cholesterol clearance by regulating Pcsk9 expression in the liver. JCI Insight 2:10 (2017).
2. Wen S et al. Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice. J Lipids 2013:908048 (2013).
序列相似性
Belongs to the peptidase S8 family.
组织特异性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
翻译后修饰
Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation.; Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein.; Phosphorylation protects the propeptide against proteolysis.
亚细胞定位
Secreted, endoplasmic reticulum,lysosome, cytoplasm.
别名
Convertase subtilisin/kexin type 9 preproprotein antibody
FH3 antibody
HCHOLA3 antibody
Hypercholesterolemia autosomal dominant 3 antibody
LDLCQ1 antibody
NARC 1 antibody
NARC-1 antibody
NARC1 antibody
Neural apoptosis regulated convertase 1 antibody
Neural apoptosis-regulated convertase 1 antibody
展开图片
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Western blot analysis of PCSK9 on PANC-1 using anti-PCSK9 antibody at 1/100 dilution.
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Western blot analysis of PCSK9 on H22 (1) and SW1990 (2) using anti-PCSK9 antibody at 1/500 dilution.
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Immunocytochemistry analysis of HepG2 cells labeling PCSK9 with Mouse anti-PCSK9 antibody (EM1701-26) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCSK9 antibody (EM1701-26) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon caner tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of HepG2 cells labeling PCSK9.
Cells were fixed and permeabilized. Then stained with the primary antibody (EM1701-26, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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