Oct4 Mouse Monoclonal Antibody [A3-C11]
Catalog# EM100306
Oct4 Mouse Monoclonal Antibody [A3-C11]
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WB
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IF-Cell
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FC
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Human
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Mouse
概述
产品名称
Oct4 Mouse Monoclonal Antibody [A3-C11]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within full length human Oct4 protein.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, FC
分子量
Predicted band size: 39 kDa
阳性对照
NCCIT cell lysate, D3 cell lysate, NCCIT.
偶联
unconjugated
克隆号
A3-C11
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IF-Cell
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1:200
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FC
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1:1000
靶点
功能
Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
背景文献
1. Oct4 pseudogenes are transcribed in cancers."Suo G., Han J., Wang X., Zhang J., Zhao Y., Zhao Y., Dai J.Biochem. Biophys. Res. Commun. 337:1047-1051(2005)
2. Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation."Wei F., Schoeler H.R., Atchison M.L.J. Biol. Chem. 282:21551-21560(2007)
3. Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1."Zhang Z., Liao B., Xu M., Jin Y.FASEB J. 21:3042-3051(2007)
序列相似性
Belongs to the POU transcription factor family. Class-5 subfamily.
组织特异性
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
翻译后修饰
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.; Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
Octamer binding transcription factor 4 antibody
MGC22487 antibody
Oct 3 antibody
Oct 4 antibody
Oct-3 antibody
Oct-4 antibody
OCT3 antibody
Oct4 antibody
Octamer binding protein 3 antibody
Octamer binding protein 4 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (EM100306) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Oct4 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 42 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM100306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NCCIT cell lysate
Lane 2: D3 cell lysate -
ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Flow cytometric analysis of NCCIT cells labeling Oct4.
Cells were fixed and permeabilized. Then stained with the primary antibody (EM100306, 1:1000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"