HLA Class 1 ABC Mouse Monoclonal Antibody [11C2]
Catalog# EM1801-10
HLA Class 1 ABC Mouse Monoclonal Antibody [11C2]
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WB
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IHC-P
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Human
概述
产品名称
HLA Class 1 ABC Mouse Monoclonal Antibody [11C2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human HLA Class 1 B aa 1-362 / 362.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 41 kDa
阳性对照
HeLa cell lysate, HepG2 cell lysate, 293T, human liver cancer tissue, human kidney tissue, mouse testis tissue, human liver tissue.
偶联
unconjugated
克隆号
11C2
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:200-1:1,000
发表文章中的应用
| IF | 查看 1 篇文献如下 |
发表文章中的种属
| Mouse | 查看 2 篇文献如下 |
靶点
功能
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
背景文献
1. Mordoh J et al. Phase II Study of Adjuvant Immunotherapy with the CSF-470 Vaccine Plus Bacillus Calmette-Guerin Plus Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor vs Medium-Dose Interferon Alpha 2B in Stages IIB, IIC, and III Cutaneous Melanoma Patients: A Single Institution, Randomized Study. Front Immunol 8:625 (2017).
2. Pavesi A et al. A 3D microfluidic model for preclinical evaluation of TCR-engineered T cells against solid tumors. JCI Insight 2(12). pii: 89762 (2017).
亚细胞定位
Cell membrane, Endoplasmic reticulum membrane .
别名
Antigen presenting molecule antibody
HLA class I histocompatibility antigen, A 1 alpha chain antibody
HLAA antibody
Leukocyte antigen class I A antibody
Major histocompatibility complex, class I, A antibody
MHC class I antigen HLA A heavy chain antibody
AS antibody
HLA B protein antibody
HLA class I histocompatibility antigen, B alpha chain antibody
HLA-B antibody
展开图片
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Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 2 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-HLA Class 1 ABC KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-10) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-10) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Neurons upregulate PD-L1 via IFN/STAT1/IRF1 to alleviate damage by CD8+ T cells in cerebral malaria
Author: Wang Yi,et al
PMID: 38715061
应用:
反应种属: Mouse
发表时间: 2024 May
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Citation
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CD8+ T cell infiltration and proliferation in the brainstem during experimental cerebral malaria
Author:
PMID: 37697956
应用: IF
反应种属: Mouse
发表时间: 2023 Sept
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Citation