Calreticulin Mouse Monoclonal Antibody [7B2]

Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (EM1701-61) at 1/5,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HL-60 cell lysate (20 µg/Lane)

Predicted band size: 48 kDa
Observed band size: 55 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-61) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (EM1701-61) at 1/50,000 dilution.

Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si Calreticulin cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 55 kDa

Exposure time: 13 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-61) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Calreticulin with Mouse anti-Calreticulin antibody (EM1701-61) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Calreticulin antibody (EM1701-61) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.

Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.