CTNNBL1 Mouse Monoclonal Antibody [5E-4F]
Catalog# EM1706-34
CTNNBL1 Mouse Monoclonal Antibody [5E-4F]
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
概述
产品名称
CTNNBL1 Mouse Monoclonal Antibody [5E-4F]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
65 kDa
阳性对照
Human CTNNBL1 recombinant protein, CTNNBL1-hIgGFc transfected HEK293 cell lysate, Hela, Jurkat, HEK293, A431, HepG2, Raji, MCF-7, human colon cancer tissue, human esophageal cancer tissue.
偶联
unconjugated
克隆号
5E-4F
产品特性
形态
Liquid
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
IF-cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
靶点
功能
CTNNBL1 (catenin, β like 1), also known as NAP (nuclear-associated protein) or P14L, is an evolutionarily conserved protein with structural homology to members of the armadillo family, including β-catenin. CTNNBL1 is expressed in a variety of tissues with highest expression levels found in heart, spleen, testis, placenta, thyroid and skeletal muscle. Localizing to the nucleus, CTNNBL1 contains a bipartite nuclear localization signal, an acidic domain, a leucine-isoleucine zipper, an acidic domain and phosphorylation sites. Via its C-terminus, CTNNBL1 is believed to play a role in apoptosis. In addition, multiple SNPs (single nucleotide polymorphisms) in the CTNNBL1 have been associated with fat mass and body mass index (BMI), suggesting a possible role for CTNNBL1 in the development of obesity.
背景文献
1. Pourfarzad F et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013).
2. Conticello SG et al. Interaction between antibody-diversification enzyme AID and spliceosome-associated factor CTNNBL1.Mol Cell. 31(4):474-84 (2008).
亚细胞定位
Nucleus.
UNIPROT
别名
5730471K09Rik antibody
Beta catenin like 1 antibody
Beta catenin like protein 1 antibody
Beta-catenin-like protein 1 antibody
C20orf33 antibody
Cadherin associated protein antibody
Catenin beta like 1 antibody
Chromosome 20 open reading frame 3 antibody
CTBL1_HUMAN antibody
CTNN protein antibody
展开图片
-
Western blot analysis of CTNNBL1 on human CTNNBL1 recombinant protein using anti-CTNNBL1 antibody at 1/1,000 dilution.
-
Western blot analysis of CTNNBL1 on HEK293 (1) and CTNNBL1-hIgGFc transfected HEK293 (2) cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution.
-
Western blot analysis of CTNNBL1 on different cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution.
Positive control: Line1: Hela Line2: Jurkat Line3: HEK293 Line4: A431 Line5: HepG2 Line6: Raji -
ICC staining CTNNBL1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.
-
Flow cytometric analysis of Hela cells with CTNNBL1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"