Skip to content

Phospho-P38 (T180 + Y182) Rabbit Polyclonal Antibody

RMB: 1600

产品规格

现在订购，【少量现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# ER2001-52

Phospho-P38 (T180 + Y182) Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Phospho-P38 (T180 + Y182) Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic phospho-peptide corresponding to residues surrounding Thr180 and Y182 of human P38.

种属反应性

Human, Mouse, Rat

验证应用

WB

分子量

Predicted band size: 41 kDa

阳性对照

NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate, HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate, C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate, PC-12 treated with UV for 1 hour cell lysate.

偶联

unconjugated

RRID

AB_3069529

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

发表文章中的应用

WB 查看 30 篇文献如下

靶点

功能

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.

背景文献

1. Deak M. et. al. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17:4426-4441(1998).

亚细胞定位

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q16539 Human

SwissProt: P47811 Mouse

SwissProt: P70618 Rat

别名

CSAID Binding Protein 1 antibody

CSAID binding protein antibody

CSAID-binding protein antibody

Csaids binding protein antibody

CSBP 1 antibody

CSBP 2 antibody

CSBP antibody

CSBP1 antibody

CSBP2 antibody

CSPB1 antibody

展开

图片

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-p38 (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 (T180 + Y182) antibody (<a href="/products/ER2001-52" style="font-weight: bold;text-decoration: underline;">ER2001-52</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 1 minute 29 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-52" style="font-weight: bold;text-decoration: underline;">ER2001-52</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-p38 (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 (T180 + Y182) antibody (ER2001-52) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate
    Lane 5: C6 cell lysate
    Lane 6: C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 1 minute 29 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-p38 (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 (T180 + Y182) antibody (<a href="/products/ER2001-52" style="font-weight: bold;text-decoration: underline;">ER2001-52</a>) at 1/2,000 dilution.<br /><br />Lane 1: PC-12 cell lysate (40 µg/Lane)<br />Lane 2: PC-12 treated with UV for 1 hour cell lysate (40 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-52" style="font-weight: bold;text-decoration: underline;">ER2001-52</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-p38 (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 (T180 + Y182) antibody (ER2001-52) at 1/2,000 dilution.

    Lane 1: PC-12 cell lysate (40 µg/Lane)
    Lane 2: PC-12 treated with UV for 1 hour cell lysate (40 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

同靶点 & 同通路的产品

metafield image

Phospho-p38 (T180) Recombinant Rabbit Monoclonal Antibody [JE77-37]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

p38 alpha Recombinant Rabbit Monoclonal Antibody [JF55-07]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-p38 (T180) Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单

请您留言

杭州华安生物技术有限公司 0571-88062880

您需要的服务
抗体咨询与购买
提交