Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody

功能

Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.

背景文献

1. Schmidt AF et al. Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. J Neuroinflammation 13:238 (2016).

2. Olympiou M et al. Systemic inflammation disrupts oligodendrocyte gap junctions and induces ER stress in a model of CNS manifestations of X-linked Charcot-Marie-Tooth disease. Acta Neuropathol Commun 4:95 (2016).

序列相似性

Belongs to the myelin basic protein family.

组织特异性

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

翻译后修饰

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.; The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).; Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.; Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

亚细胞定位

Myelin membrane, Nucleus.

UNIPROT

SwissProt: P04370 Mouse

别名