Granzyme B Rabbit Polyclonal Antibody
Catalog# IRS054
Granzyme B Rabbit Polyclonal Antibody
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mIHC
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Mouse
概述
产品名称
Granzyme B Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human Granzyme B aa 50-247.
种属反应性
Mouse
验证应用
mIHC
分子量
Predicted band size: 28 kDa
阳性对照
Mouse osteosarcoma tissue, mouse spleen tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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mIHC
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1:100
靶点
功能
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse . It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death . Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis .
背景文献
1. Hameed A. et. al. Characterization of three serine esterases isolated from human IL-2 activated killer cells. J. Immunol. 141:3142-3147(1988).
亚细胞定位
Secreted.
UNIPROT
别名
C11 antibody
Cathepsin G like 1 antibody
Cathepsin G-like 1 antibody
CCPI antibody
CGL 1 antibody
CGL1 antibody
CSP B antibody
CSPB antibody
CTLA 1 antibody
CTLA-1 antibody
展开图片
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mIHC analysis of mouse osteosarcoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Granzyme B antibody (IRS054) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Granzyme B antibody (IRS054) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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