Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS011
Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
概述
产品名称
Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Cytokeratin 19 aa 348-400 / 400.
种属反应性
Human
验证应用
mIHC
阳性对照
Human cholangiocarcinoma tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
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1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.
靶点
功能
Keratin, type I cytoskeletal 19 also known as cytokeratin-19 (CK-19) or keratin-19 (K19) is a 40 kDa protein that in humans is encoded by the KRT19 gene. Keratin 19 is a type I keratin. Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis.
背景文献
1. Guye P et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016).
2. Cui M et al. PTEN is a potent suppressor of small cell lung cancer. Mol Cancer Res 12:654-9 (2014).
序列相似性
Belongs to the intermediate filament family.
组织特异性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
亚细胞定位
Cytoplasm
UNIPROT
别名
40 kDa keratin intermediate filament antibody
CK 19 antibody
CK-19 antibody
CK19 antibody
Cytokeratin 19 antibody
Cytokeratin-19 antibody
K19 antibody
K1C19_HUMAN antibody
K1CS antibody
Keratin 19 antibody
展开图片
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mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 19 antibody (IRS011) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), FOXP3, CK19 (IRS011), PD-L1, CD4 (IRS005), CD163 (IRS002) and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human endometrial cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CK19 (IRS011), FOXP3 and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with CHGA, CD31 (IRS023), CK19 (IRS011) and Somatostatin 28 antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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