HRP Conjugated Rabbit anti-Chicken IgY polyclonal Antibody
Catalog# HA1026
HRP Conjugated Rabbit anti-Chicken IgY polyclonal Antibody
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ELISA
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WB
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Chicken IgY
概述
产品名称
HRP Conjugated Rabbit anti-Chicken IgY polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein.
种属反应性
Chicken IgY
验证应用
ELISA, WB
偶联
HRP
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.5%BSA, 50% Glycerol.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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ELISA
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1:5,000-1:10,000
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WB
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1:500-1:2,000
靶点
功能
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
背景文献
暂无
别名
Immunoglobulin of yolk
图片
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Western blot analysis of Rabbit anti Chicken IgY-HRP on Chicken IgY lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Chicken IgY-HRP Secondary Antibody (HA1026) at 1:500 (Lane 1),1:1,000 (Lane 2)dilution was used for 2 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"