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PE Conjugated Goat anti-mouse IgG polyclonal Antibody

RMB: 450

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Catalog# HA1015

PE Conjugated Goat anti-mouse IgG polyclonal Antibody

Application

  • FC

  • IF

Reactivity

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

PE Conjugated Goat anti-mouse IgG polyclonal Antibody

抗体类型

Goat Polyclonal Antibody

免疫原

Mouse IgG (H+L).

产品特异性

To minimize cross-reactivity, the PE conjugated goat anti-mouse IgG (H+L) antibodies have been highly cross-adsorbed against human IgG.

种属反应性

Mouse

验证应用

FC, IF

偶联

PE

产品特性

形态

Liquid

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 1%BSA, 50%Glycerol.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • FC

  • 1:100-1000

  • IF

  • 1:100-1000

发表文章中的应用

IF 查看 1 篇文献如下

发表文章中的种属

Pig 查看 1 篇文献如下

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Phycoerythrin (PE) is a fluorescent protein isolated and purified from red algae. Because of its strong fluorescence, good light absorption performance and high quantum yield, it has a wide excitation and emission range in the visible spectrum region, so in the fluorescence of immunofluorescence, immunohistochemistry, flow cytometry and other antibodies Marking and in vivo imaging have a wide range of applications.

背景文献

暂无

图片

  • Flow cytometric analysis of HeLa cells labeling beta Tubulin.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a PE conjugate-Goat anti-Mouse IgG Secondary antibody (<a href="/products/HA1015" style="font-weight: bold;text-decoration: underline;">HA1015</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling beta Tubulin.

    Cells were fixed and permeabilized. Then stained with the primary antibody (M1305-2, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a PE conjugate-Goat anti-Mouse IgG Secondary antibody (HA1015) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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  1. IF
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