Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
Catalog# HA601340
Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
-
FC
-
Human
概述
产品名称
Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within
种属反应性
Human
验证应用
FC
分子量
Predicted band size: 29 kDa
阳性对照
Human peripheral blood granulocytes.
偶联
unconjugated
克隆号
PSH04-95
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
FC
-
1:1,000
靶点
功能
CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens.
背景文献
1. Liu X et al. CD16(+) fibroblasts foster a trastuzumab-refractory microenvironment that is reversed by VAV2 inhibition. Cancer Cell. 2022 Nov
2. Cocker ATH et al. The CD56-CD16+ NK cell subset in chronic infections. Biochem Soc Trans. 2023 Jun
亚细胞定位
Cell membrane, Secreted.
UNIPROT
别名
CD 16 antibody
CD 16a antibody
CD16 antibody
CD16a antibody
CD16a antigen antibody
CD16B antibody
CD16b antigen antibody
Fc fragment of IgG antibody
Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
Fc fragment of IgG low affinity IIIa receptor antibody
展开图片
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Flow cytometric analysis of human peripheral blood granulocytes labeling Human CD16.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601340, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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