Lin28B Mouse Monoclonal Antibody [A2-4-5]
Catalog# M1312-1
Lin28B Mouse Monoclonal Antibody [A2-4-5]
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WB
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IF-Cell
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IHC-P
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Human
概述
产品名称
Lin28B Mouse Monoclonal Antibody [A2-4-5]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human Lin28B aa 73-250 / 250.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 27 kDa
阳性对照
JAR cell lysate, NCCIT, human placenta tissue.
偶联
unconjugated
克隆号
A2-4-5
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2a
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:100-1:200
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IHC-P
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1:1,000
靶点
功能
LIN28 is thought to regulate the self-renewal of stem cells. In nematodes, the LIN28 homolog lin-28 is a heterochronic gene that determines the onset of early larval stages of developmental events in Caenorhabditis elegans, by regulating the self-renewal of nematode stem cells in the skin (called seam cells) and vulva (called VPCs) during development. In mice, LIN28 is highly expressed in mouse embryonic stem cells and during early embryogenesis.It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Lin28B is high expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC).
背景文献
1. "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation." Heo I., Joo C., Kim Y.-K., Ha M., Yoon M.-J., Cho J., Yeom K.-H., Han J., Kim V.N. Cell 138:696-708(2009)
2. "Lin28 promotes transformation and is associated with advanced human malignancies." Viswanathan S.R., Powers J.T., Einhorn W., Hoshida Y., Ng T.L., Toffanin S., O\'Sullivan M., Lu J., Phillips L.A., Lockhart V.L., Shah S.P., Tanwar P.S., Mermel C.H., Beroukhim R., Azam M., Teixeira J., Meyerson M., Hughes T.P. Daley G.Q. Nat. Genet. 41:843-848(2009)
3. "The Lin28 cold-shock domain remodels pre-let-7 microRNA." Mayr F., Schutz A., Doge N., Heinemann U. Nucleic Acids Res. 40:7492-7506(2012)
序列相似性
Belongs to the lin-28 family.
组织特异性
Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver. Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas.
亚细胞定位
Cytoplasm, nucleus
UNIPROT
别名
CSDD 2 antibody
CSDD2 antibody
FLJ16517 antibody
Lin 28 homolog B (C. elegans) antibody
Lin 28.2 antibody
Lin-28B antibody
LIN28B antibody
LN28B_HUMAN antibody
Protein lin-28 homolog B antibody
图片
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☑ Relative expression (RE)
Western blot analysis of Lin28B on different lysates with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (negative)
Lane 2: JAR cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1312-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining Lin28B in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. and counterstained with DAPI in order to highlight the nucleus (blue).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1312-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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