Skip to content

SIRT6 Recombinant Rabbit Monoclonal Antibody [PSH10-99]

RMB: 699 特惠 1500

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# HA723266

SIRT6 Recombinant Rabbit Monoclonal Antibody [PSH10-99]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Monkey

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

SIRT6 Recombinant Rabbit Monoclonal Antibody [PSH10-99]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human SIRT6 aa 1-355.

种属反应性

Human, Mouse, Rat, Monkey

验证应用

WB, IHC-P

分子量

Predicted band size: 39 kDa

阳性对照

HeLa cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, mouse testis tissue.

偶联

unconjugated

克隆号

PSH10-99

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 1 篇文献如下

靶点

功能

Sirtuin 6 (SIRT6 or Sirt6) is a stress responsive protein deacetylase and mono-ADP ribosyltransferase enzyme encoded by the SIRT6 gene. In laboratory research, SIRT6 appears to function in multiple molecular pathways related to aging, including DNA repair, telomere maintenance, glycolysis and inflammation. SIRT6 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein.

背景文献

1. Guo Z et al. SIRT6 in Aging, Metabolism, Inflammation and Cardiovascular Diseases. Aging Dis. 2022 Dec

2. Li B et al. SIRT6-regulated macrophage efferocytosis epigenetically controls inflammation resolution of diabetic periodontitis. Theranostics. 2023 Jan

亚细胞定位

Nucleus, Chromosome, telomere, Endoplasmic reticulum.

UNIPROT

SwissProt: Q8N6T7 Human

SwissProt: P59941 Mouse

Entrez Gene: 299638 Rat

别名

2810449N18Rik antibody

AI043036 antibody

Mono ADP ribosyltransferase sirtuin 6 antibody

NAD-dependent protein deacetylase sirtuin-6 antibody

Regulatory protein SIR2 homolog 6 antibody

Regulatory protein SIR2 homolog antibody

SIR2 like 6 antibody

SIR2 like protein 6 antibody

Sir2 related protein type 6 antibody

SIR2-like protein 6 antibody

展开

图片

  • Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (<a href="/products/HA723266" style="font-weight: bold;text-decoration: underline;">HA723266</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HCT 116 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: MEF cell lysate (20 µg/Lane)<br />Lane 5: C6 cell lysate (20 µg/Lane)<br />Lane 6: PC-12 cell lysate (20 µg/Lane)<br />Lane 7: COS-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 39 kDa<br />Observed band size: 36/42 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723266" style="font-weight: bold;text-decoration: underline;">HA723266</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (HA723266) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HCT 116 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: MEF cell lysate (20 µg/Lane)
    Lane 5: C6 cell lysate (20 µg/Lane)
    Lane 6: PC-12 cell lysate (20 µg/Lane)
    Lane 7: COS-1 cell lysate (20 µg/Lane)

    Predicted band size: 39 kDa
    Observed band size: 36/42 kDa

    Exposure time: 46 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SIRT6 antibody (<a href="/products/HA723266" style="font-weight: bold;text-decoration: underline;">HA723266</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723266" style="font-weight: bold;text-decoration: underline;">HA723266</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SIRT6 antibody (HA723266) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723266) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (<a href="/products/HA723266" style="font-weight: bold;text-decoration: underline;">HA723266</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-SIRT6 KO cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 39 kDa<br />Observed band size: 39 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723266" style="font-weight: bold;text-decoration: underline;">HA723266</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (HA723266) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-SIRT6 KO cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单