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Nrf2 Rabbit Polyclonal Antibody

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Catalog# R1312-8

Nrf2 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Nrf2 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

KLH conjugated synthetic peptide derived from human Nrf2: 401-500/605

种属反应性

Human, Mouse

验证应用

WB, IHC-P, IF-Tissue

分子量

Predicted band size: 68 kDa

偶联

unconjugated

RRID

AB_3073268

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

存储缓冲液

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:100-1000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

靶点

功能

The Nrf2 antioxidant response pathway is "the primary cellular defense against the cytotoxic effects of oxidative stress." Among other effects, NFE2L2 increases the expression of several antioxidant enzymes. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress, and dimethyl fumarate significantly reduced the progression of disability in multiple sclerosis. Under normal or unstressed conditions, Nrf2 is kept in the cytoplasm by a cluster of proteins that degrade it quickly. Under oxidative stress, Nrf2 is not degraded, but instead travels to the nucleus where it binds to a DNA promoter and initiates transcription of antioxidative genes and their proteins.

背景文献

1. "Structure of the Keap1:Nrf2 interface provides mechanistic insight into Nrf2 signaling." Lo S.-C., Li X., Henzl M.T., Beamer L.J., Hannink M. EMBO J. 25:3605-3617(2006)

2. "Acetylation-deacetylation of the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) regulates its transcriptional activity and nucleocytoplasmic localization." Kawai Y., Garduno L., Theodore M., Yang J., Arinze I.J. J. Biol. Chem. 286:7629-7640(2011)

3. "Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing." Kaitsuka T., Tomizawa K., Matsushita M. EMBO Rep. 12:673-681(2011)

序列相似性

Belongs to the bZIP family. CNC subfamily.

组织特异性

Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

翻译后修饰

Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated (By similarity).; Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.; Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.; Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.

亚细胞定位

Cytoplasm, Nucleus

UNIPROT

SwissProt: Q16236 Human

SwissProt: Q60795 Mouse

别名

erythroid derived 2 antibody

HEBP1 antibody

like 2 antibody

NF E2 related factor 2 antibody

NF-E2-related factor 2 antibody

NF2L2_HUMAN antibody

NFE2 related factor 2 antibody

NFE2-related factor 2 antibody

Nfe2l2 antibody

Nrf 2 antibody

展开

图片

  • Hep-G2 (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

    Hep-G2 (H) cells were treated with or without MG-132 (10 μM) for 10h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 polyclonal antibody, unconjugated (R1312-8) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (<a href="/products/R1312-8" style="font-weight: bold;text-decoration: underline;">R1312-8</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (R1312-8) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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