Human IL-18 DTSet ELISA 检测试剂盒
概述
产品名称
Human IL-18 DTSet ELISA 检测试剂盒
产品类型
DTSet Elisa Kit

pg/ml | O.D. | Average | Corrected | |
---|---|---|---|---|
0.00 | 0.0282 | 0.0273 | 0.0278 | |
62.50 | 0.1634 | 0.1661 | 0.1648 | 0.1370 |
125.00 | 0.2907 | 0.2849 | 0.2878 | 0.2601 |
250.00 | 0.5127 | 0.5121 | 0.5124 | 0.4847 |
500.00 | 0.9234 | 0.9091 | 0.9163 | 0.8885 |
1000.00 | 1.7292 | 1.7016 | 1.7154 | 1.6877 |
2000.00 | 2.7903 | 2.9158 | 2.8531 | 2.8253 |
4000.00 | 4.0936 | 4.1241 | 4.1089 | 4.0811 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Human
检测方法
比色法
实验检测时长
120min
标准曲线梯度
7点/3倍
孵育次数
2
加样体积
50 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
蛋白名
IL-18
功能
白细胞介素18(IL-18),也称为干扰素-γ诱导因子(IGIF)和IL-1γ,是一种细胞因子,与IL-12具有生物学活性,与IL-1蛋白家族具有结构相似性。IL-18最初是从肝细胞克隆而来的,后来被证明由单核细胞/巨噬细胞、成骨细胞和角质形成细胞表达。半胱天冬酶-1(IL-1β转换酶)与前IL-18到IL-18的生理加工有关。与IL-12类似,人IL-18已被证明可以增强PBMC培养物中的NK细胞活性。还发现人IL-18可诱导IFN-γ和GM-CSF的产生,同时抑制PBMC产生IL-10。在富集的人T细胞上,人IL-18可以通过IL-2依赖性途径增强Th1细胞因子的产生并刺激细胞增殖。在小鼠系统中,IL-18已被证明是激活Th1的共刺激因子,但不是Th2细胞。发现IL-18选择性增强Th1细胞的FasL介导的细胞毒性,但不能增强Th0或Th2细胞的细胞毒性。IL-18也被证明可以诱导活化的B细胞产生抑制IgE产生的IFN-γ。
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