Human IFN-γ DTSet ELISA 检测试剂盒
概述
产品名称
Human IFN-γ DTSet ELISA 检测试剂盒
产品类型
DTSet Elisa Kit

pg/ml | O.D. | Average | Corrected | |
---|---|---|---|---|
0.00 | 0.0133 | 0.0117 | 0.0125 | |
9.38 | 0.1031 | 0.1072 | 0.1052 | 0.0927 |
18.75 | 0.1913 | 0.1975 | 0.1944 | 0.1819 |
37.50 | 0.3300 | 0.3718 | 0.3509 | 0.3384 |
75.00 | 0.7144 | 0.7221 | 0.7183 | 0.7058 |
150.00 | 1.2980 | 1.3150 | 1.3065 | 1.2940 |
300.00 | 2.2590 | 2.3650 | 2.3120 | 2.2995 |
600.00 | 3.6480 | 3.6260 | 3.6370 | 3.6245 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Human
检测方法
比色法
实验检测时长
120min
标准曲线梯度
7点/3倍
孵育次数
2
加样体积
50 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
蛋白名
IFN-γ
功能
干扰素-γ(IFN-γ),也称为II型或免疫干扰素,发挥广泛的免疫调节活性,被认为是原型促炎细胞因子。成熟的人的IFN-γ以20-25kDa可变糖基化亚基的非共价连接的同源二聚体存在。它与恒河猴IFN-γ共享90%的氨基酸(aa)序列同一性,与牛、犬、马、猫和猪IFN-γ共享59%-64%氨基酸序列同一性,与棉花大鼠、小鼠和大鼠IFN-γ共享37%-43%氨基酸序列同一性。IFN-γ二聚体与IFN-γ RI(α亚基)结合,然后与IFN-γ-RII(β亚基)相互作用,形成两个α和两个β亚基的功能受体复合物。IFN-γ RII的加入增加了配体的结合亲和力和信号转导的效率。IFN-γ由多种免疫细胞在炎症条件下产生,特别是由T细胞和NK细胞产生。它通过促进Th1细胞的发育和活化,单核细胞和巨噬细胞的化学吸引和活化,抗原呈递分子的上调以及B细胞中的免疫球蛋白类别转换,在宿主防御中起关键作用。它还表现出抗病毒、抗增殖和凋亡作用。此外,IFN-γ通过促进调节性T细胞的发育和抑制Th17细胞分化来发挥抗炎介质的作用。IFN-γ的多效性作用有助于动脉粥样硬化的多个方面的发展。
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