iFluor™ 488 Conjugated Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07]
Catalog# HA720139F
iFluor™ 488 Conjugated Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07]
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IF-Tissue
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
iFluor™ 488 Conjugated Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse Cytokeratin 18 aa 200-423.
种属反应性
Human, Mouse, Rat
验证应用
IF-Tissue, IF-Cell
分子量
Predicted band size: 48 kDa
阳性对照
Human kidney tissue, SK-Br-3.
偶联
iFluor™ 488
克隆号
SZ80-07
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Tissue
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1:50
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IF-Cell
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1:100
靶点
功能
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.
背景文献
1. Kuramoto G. et. al. Preventive effect of oral mucosal epithelial cell sheets on intrauterine adhesions. Hum Reprod 30:406-16 (2015).
2. Huang YS. et. al. RGD surface functionalization of the hydrophilic acrylic intraocular lens material to control posterior capsular opacification. PLoS One 9:e114973 (2014).
亚细胞定位
Cytoplasm, Nucleus.
别名
Cell proliferation inducing gene 46 protein antibody
Cell proliferation inducing protein 46 antibody
Cell proliferation-inducing gene 46 protein antibody
CK 18 antibody
CK-18 antibody
CK18 antibody
CYK 18 antibody
CYK18 antibody
Cytokeratin 18 antibody
Cytokeratin endo B antibody
展开图片
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Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 18 (HA720139F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 18 (HA720139F, green) at 1/50 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. -
Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 18 with Rabbit anti-Cytokeratin 18 antibody (HA720139F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 18 antibody (HA720139F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"