SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]
Catalog# ET1610-77
SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human SCF aa 36-85 / 273.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Tissue, IHC-P
分子量
Predicted band size: 31 kDa
阳性对照
Raji cell lysate, COLO205 cell lysate, HT-29 cell lysate, A549 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human prostate cancer tissue, human kidney tissue.
偶联
unconjugated
克隆号
SC61-06
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:1:1,000
发表文章中的应用
| IHC | 查看 1 篇文献如下 |
发表文章中的种属
| Mouse | 查看 1 篇文献如下 |
靶点
功能
Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells.
背景文献
1. Xiang FL et al. Cardiac-specific overexpression of human stem cell factor promotes epicardial activation and arteriogenesis after myocardial infarction. Circ Heart Fail 7:831-42 (2014).
2. Nishikori Y et al. The role of mast cells in cutaneous wound healing in streptozotocin-induced diabetic mice. Arch Dermatol Res 306:823-35 (2014).
序列相似性
Belongs to the SCF family.
翻译后修饰
A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.; Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.; A soluble form exists as a cleavage product of the extracellular domain.
亚细胞定位
Cell membrane, Cytoplasm, Secreted.
别名
C kit ligand antibody
C-kit ligand antibody
Ckit ligand antibody
DCUA antibody
DFNA69 antibody
DKFZp686F2250 antibody
familial progressive hyperpigmentation 2 antibody
FPH2 antibody
FPHH antibody
KIT ligand antibody
展开图片
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Western blot analysis of SCF on different lysates with Rabbit anti-SCF antibody (ET1610-77) at 1/2,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: COLO205 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)
Lane 6: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 35 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Wei-Tong-Xin ameliorated cisplatin-induced mitophagy and apoptosis in gastric antral mucosa by activating the Nrf2/HO-1 pathway
Author:
PMID: 36806345
应用: IHC
反应种属: Mouse
发表时间: 2023 May
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Citation
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