CD9 Recombinant Rabbit Monoclonal Antibody [PSH0-95]
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Catalog# HA721533
CD9 Recombinant Rabbit Monoclonal Antibody [PSH0-95]
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WB
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IHC-P
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Human
概述
产品名称
CD9 Recombinant Rabbit Monoclonal Antibody [PSH0-95]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CD9 aa 196-228 / 228.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 25 kDa
阳性对照
HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, SW480 cell lysate, BT-20 cell lysate, human colon carcinoma tissue, human lung carcinoma tissue.
偶联
unconjugated
克隆号
PSH0-95
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000-1:5,000
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IHC-P
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1:200-1:1,000
靶点
功能
CD9 is a gene encoding a protein that is a member of the transmembrane 4 superfamily also known as the tetraspanin family. It is a cell surface glycoprotein that consists of four transmembrane regions and has two extracellular loops that contain disulfide bonds which are conserved throughout the tetraspanin family. Also containing distinct palmitoylation sites that allows CD9 to interact with lipids and other proteins. Tetraspanin proteins are involved in a multitude of biological processes such as adhesion, motility, membrane fusion, signaling and protein trafficking. Tetraspanins play a role in many biological processes because of their ability to interact with many different proteins including interactions between each other. Their distinct palmitoylation sites allow them to organize on the membrane into tetraspanin-enriched microdomains (TEM). These TEMs are thought to play a role in many cellular processes including exosome biogenesis. CD9 is commonly used as a marker for exosomes as it is contained on their surface.
背景文献
1. Mathieu M et al. Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nat Commun. 2021 Jul
2. Cho JH et al. CD9 induces cellular senescence and aggravates atherosclerotic plaque formation. Cell Death Differ. 2020 Sep
亚细胞定位
Cell membrane, Membrane, Secreted, extracellular exosome.
UNIPROT
别名
Tetraspanin 29 antibody
5H9 antibody
5H9 antigen antibody
Antigen defined by monoclonal antibody 602 29 antibody
Antigen defined by monoclonal antibody 60229 antibody
BA-2/p24 antigen antibody
BA2 antibody
BTCC 1 antibody
BTCC1 antibody
CD9 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: MCF7 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 20 kDa
Exposure time: Lane 1-4 (left): 53 seconds; Lane 1-4 (right): 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: SW480 cell lysate (10 µg/Lane)
Lane 3: BT-20 cell lysate (10 µg/Lane)
Lane 4: MCF7 cell lysate (10 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 20 kDa
Exposure time: 42 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/2,000 dilution.
Lane 1: HepG2-si NT cell lysate
Lane 2: HepG2-si CD9 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 20 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CD9 antibody (HA721533) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721533) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-CD9 antibody (HA721533) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721533) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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