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Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02]

RMB: 900 特惠 1500

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Catalog# ET1611-28

Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Angiopoietin 1 aa 249-300 / 498.

种属反应性

Human, Mouse, Rat

验证应用

WB

分子量

Predicted band size: 58 kDa

阳性对照

U-87 MG cell lysate, HeLa cell lysate, K-562 cell lysate, C6 cell lysate, RAW264.7 cell lysate.

偶联

unconjugated

克隆号

SN67-02

RRID

AB_3070005

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

发表文章中的应用

WB 查看 1 篇文献如下
IF 查看 1 篇文献如下

发表文章中的种属

Pig 查看 1 篇文献如下

靶点

功能

Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. During pregnancy, angiopoietins act complementary to the VEGF system and contribute to endothelial cell survival and the remodeling of vessels. Few studies have examined the role of angiopoietins in human pregnancy complications like preeclampsia and intrauterine growth restriction (IUGR).

背景文献

1. Stewart N & Chade AR Renoprotective effects of hepatocyte growth factor in the stenotic kidney. Am J Physiol Renal Physiol 304:F625-33 (2013).

2. Mohamad O et al. Vector-free and transgene-free human iPS cells differentiate into functional neurons and enhance functional recovery after ischemic stroke in mice. PLoS One 8:e64160 (2013).

翻译后修饰

Glycosylated.

亚细胞定位

Secreted.

UNIPROT

SwissProt: Q15389 Human

SwissProt: O08538 Mouse

SwissProt: O35460 Rat

别名

AGP 1 antibody

AGP1 antibody

AGPT antibody

ANG 1 antibody

ANG antibody

ANG-1 antibody

ANG1 antibody

Angiopoietin-1 antibody

Angiopoietin1 antibody

ANGP1_HUMAN antibody

展开

图片

  • Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (<a href="/products/ET1611-28" style="font-weight: bold;text-decoration: underline;">ET1611-28</a>) at 1/1,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: RAW264.7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-28" style="font-weight: bold;text-decoration: underline;">ET1611-28</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (ET1611-28) at 1/1,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: C6 cell lysate
    Lane 5: RAW264.7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 65 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (<a href="/products/ET1611-28" style="font-weight: bold;text-decoration: underline;">ET1611-28</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD Angiopoietin 1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-28" style="font-weight: bold;text-decoration: underline;">ET1611-28</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (ET1611-28) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD Angiopoietin 1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 65 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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