Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]
Catalog# HA601028
Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]
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WB
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IHC-P
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Human
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Mouse
概述
产品名称
Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within human Topoisomerase II alpha aa1480-1531.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
174/178/182 kDa
阳性对照
A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human breast carcinoma tissue.
偶联
unconjugated
克隆号
A3F12
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:200
发表文章中的应用
| WB | 查看 1 篇文献如下 |
靶点
功能
DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene. Topoisomerase IIα relives topological DNA stress during transcription, condenses chromosomes, and separates chromatids. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several chemotherapy agents and a variety of mutations in this gene have been associated with the development of drug resistance.[citation needed] Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. TOP2A has been shown to interact with SMURF2, HDAC1, CDC5L, Small ubiquitin-related modifier 1 and P53.
背景文献
1. Wyles J.P. et. al. Nuclear interactions of topoisomerase II alpha and beta with phospholipid scramblase 1. Nucleic Acids Res. 35:4076-4085(2007).
亚细胞定位
Cytoplasm, Nucleus.
别名
alpha isozyme antibody
ATP hydrolyzing DNA topoisomerase II alfa antibody
DNA gyrase antibody
DNA topoisomerase (ATP hydrolyzing) antibody
DNA topoisomerase 2 alpha antibody
DNA topoisomerase 2-alpha antibody
DNA topoisomerase II 170 kD antibody
DNA topoisomerase II alpha isozyme antibody
DNA topoisomerase II antibody
DNA Topoisomerase2 antibody
展开图片
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Western blot analysis of Topoisomerase II alpha on different cell lysates with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 174 kDa
Observed band size: 174 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601028) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601028) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Quantitative acetylome analysis reveals involvement of glucosyltransferase acetylation in Streptococcus mutans biofilm formation
Author:
PMID: 33185947
应用: WB
反应种属:
发表时间: 2021 Apr
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Citation
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