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Human Calprotectin (S100A8&S100A9) Protein

RMB: 3750

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Catalog# HA210041

Human Calprotectin (S100A8&S100A9) Protein

Product Information

Product name

Human Calprotectin (S100A8&S100A9) Protein

Catalog No.

HA210041

Predicted molecular mass

The protein has a predicted MW of 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Tris-Bis PAGE result.

Protein construction description

Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus. It contains Met1-Glu93(S100A8)&Thr2-Pro114(S100A9).[Accession | P05109(S100A8)&P06702(S100A9)]

Accession

P05109(S100A8)&P06702(S100A9)

Source

E.coli

Purity

> 95% as determined by Tris-Bis PAGE

Endotoxin

Less than 1EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.

Species

Human

Background

Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.

Usage guide

Storage

-20 to -80°C for 12 months as supplied from date of receipt.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

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