Human FGF-2 DTSet ELISA 检测试剂盒
概述
产品名称
Human FGF-2 DTSet ELISA 检测试剂盒
产品类型
DTSet Elisa Kit

pg/ml | O.D. | Average | Corrected | |
---|---|---|---|---|
0.00 | 0.0704 | 0.0650 | 0.0677 | |
15.63 | 0.1258 | 0.1343 | 0.1301 | 0.0624 |
31.25 | 0.1970 | 0.1986 | 0.1978 | 0.1301 |
62.50 | 0.3094 | 0.3354 | 0.3224 | 0.2547 |
125.00 | 0.5583 | 0.5755 | 0.5669 | 0.4992 |
250.00 | 1.0490 | 1.0750 | 1.0620 | 0.9943 |
500.00 | 1.9550 | 1.9820 | 1.9685 | 1.9008 |
1000.00 | 3.4760 | 3.3670 | 3.4215 | 3.3538 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Human
检测方法
比色法
实验检测时长
120min
标准曲线梯度
7点/3倍
孵育次数
2
加样体积
50 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
蛋白名
FGF-2
功能
FGF 碱性/FGF2/bFGF 是一种生长因子,在血管生成、伤口愈合、组织修复、学习和记忆以及心脏、骨骼和大脑的形态发生中起作用。它响应炎症刺激和许多肿瘤而上调。FGF basic/FGF2/bFGF与FGFR1c和2c结合。其生物活性由许多其他结合伴侣调节,包括肝素、整合素α V β 3、可溶性FGFR1、FGF-BP、游离神经节苷脂、血小板反应素、戊曲辛3/TSG-14、纤维蛋白原、α-2-巨球蛋白、PDGF和CXCL4/PF4。这些分子充当细胞共受体或粘附伙伴、细胞外基质诱饵或储库,以及可溶性清除剂或伴侣。特别是,FGF碱性/ FGF2 / bFGF与细胞表面硫酸乙酰肝素蛋白聚糖(HSPG)的相互作用是FGF受体结合和活化所必需的。
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