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Collagen II Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1906-48

Collagen II Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Rabbit

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Collagen II Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

KLH conjugated synthetic peptide derived from human Collagen II 1201-1300/1487

种属反应性

Human, Rabbit

验证应用

WB, IHC-P, IF-Tissue

分子量

117 KDa

阳性对照

Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

偶联

unconjugated

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

存储缓冲液

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

靶点

功能

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

背景文献

暂无

亚细胞定位

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P02458 Human

别名

Alpha 1 type II collagen antibody

Alpha-1 type II collagen antibody

AOM antibody

Cartilage collagen antibody

Chondrocalcin antibody

CO2A1_HUMAN antibody

COL11A3 antibody

Col2a1 antibody

Collagen 2 antibody

Collagen II alpha 1 polypeptide antibody

展开

图片

  • Sample: <br />293T (human)Cell Lysate at 40 ug<br />A549 (human)Cell Lysate at 40 ug<br />MCF-7 (human)Cell Lysate at 40 ug<br />HepG2 (human)Cell Lysate at 40 ug<br />Placenta (mouse) Lysate at 40 ug<br />MG63 (human)Cell Lysate at 40 ug<br />Primary: Anti- Collagen II (<a href="/products/ER1906-48" style="font-weight: bold;text-decoration: underline;">ER1906-48</a>) at 1/300 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 117 kD<br />Observed band size: 120 kD

    Sample:
    293T (human)Cell Lysate at 40 ug
    A549 (human)Cell Lysate at 40 ug
    MCF-7 (human)Cell Lysate at 40 ug
    HepG2 (human)Cell Lysate at 40 ug
    Placenta (mouse) Lysate at 40 ug
    MG63 (human)Cell Lysate at 40 ug
    Primary: Anti- Collagen II (ER1906-48) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 117 kD
    Observed band size: 120 kD

  • Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(<a href="/products/ER1906-48" style="font-weight: bold;text-decoration: underline;">ER1906-48</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(ER1906-48) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(<a href="/products/ER1906-48" style="font-weight: bold;text-decoration: underline;">ER1906-48</a>) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37℃. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

    Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(ER1906-48) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37℃. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC
  3. IHC-P
  4. ICC
  5. IF

Alternative Products

metafield image

Collagen II Recombinant Rabbit Monoclonal Antibody [PSH06-62]

Application: WB,IHC-P,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

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