SMCC Recombinant Rabbit Monoclonal Antibody [PSH06-06]
Catalog# HA722505
SMCC Recombinant Rabbit Monoclonal Antibody [PSH06-06]
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ELISA
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Species independent
概述
产品名称
SMCC Recombinant Rabbit Monoclonal Antibody [PSH06-06]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Fusion protein With SMCC.
种属反应性
Species independent
验证应用
ELISA
偶联
unconjugated
克隆号
PSH06-06
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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ELISA
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1:20,000
靶点
功能
Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a heterobifunctional amine-to-sulfhydryl crosslinker, which contains two reactive groups at opposite ends: N-hydroxysuccinimide-ester and maleimide, reactive with amines and thiols respectively. SMCC is often used in bioconjugation to link proteins with other functional entities (fluorescent dyes, tracers, nanoparticles, cytotoxic agents). For example, a targeted anticancer agent – trastuzumab emtansine (antibody-drug conjugate containing an antibody trastuzumab chemically linked to a highly potent drug DM-1) – is prepared using SMCC reagent. In a pharmacokinetics study, the CX-DM1-containing ADCs exhibited stability comparable to that of SMCC-DM1-containing ADCs. SMCC is a classic noncleavable linker that has been employed in Kadcyla. However, SMCC-based conjugates are still limited due to their instability in the circulatory system and hydrophobic properties.
背景文献
1. Lambert J.M., Chari R.V. Ado-trastuzumab emtansine (T-DM1): an antibody-drug conjugate (ADC) for HER2− positive breast cancer. J Med Chem. 2014;57:6949–6964
2. Dovgan I., Kolodych S., Koniev O., Wagner A. 2-(Maleimidomethyl)-1,3-dioxanes (MD): a serum-stable self-hydrolysable hydrophilic alternative to classical maleimide conjugation. Sci Rep. 2016;6:30835
别名
SMCC antibody
Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate antibody
图片
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Indirect ELISA analysis of SMCC was performed by coating wells of a 96-well plate with 50 µL per well of different SMCC conjugations diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of SMCC monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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