MMAF Mouse Monoclonal Antibody [A5G11]
Catalog# HA600035
MMAF Mouse Monoclonal Antibody [A5G11]
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ELISA
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Species independent
概述
产品名称
MMAF Mouse Monoclonal Antibody [A5G11]
抗体类型
Mouse Monoclonal Antibody
免疫原
MMAF coupled with OVA.
种属反应性
Species independent
验证应用
ELISA
偶联
unconjugated
克隆号
A5G11
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4)
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
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ELISA
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1:5,000-1:20,000
靶点
功能
Monomethyl auristatin F (MMAF) is a synthetic antineoplastic agent. It is part of the approved drug belantamab mafodotin in multiple myeloma and some experimental anti-cancer antibody-drug conjugates such as vorsetuzumab mafodotin and SGN-CD19A. In International Nonproprietary Names for MMAF-antibody-conjugates, the name mafodotin refers to MMAF plus its attachment structure to the antibody. Monomethyl auristatin F is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. It is linked to an antibody with high affinity to structures on cancer cells, causing MMAF to accumulate in such cells. MMAF is actually desmethyl-auristatin F; that is, the N-terminal amino group has only one methyl substituent instead of two as in auristatin F itself.
背景文献
1. Yu-Tzu Tai. et. al. Novel anti–B-cell maturation antigen antibody-drug conjugate (GSK2857916) selectively induces killing of multiple myeloma.Blood. 2014 May 15; 123(20): 3128–3138. doi: 10.1182/blood-2013-10-535088. PMID: 24569262.
别名
MMAF antibody
Monomethyl auristatin F antibdy
图片
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The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA. -
Down ELISA Binding Assay of anti-MMAF Antibody A5G11(HA600035) to MMAF-ADC and MMAF-BSA. The mouse mAb works fine with ELISA assay for measuring MMAF derivative ADC.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"