Cytokeratin 7 Mouse Monoclonal Antibody [3-G3-D8]
Catalog# EM0702
Cytokeratin 7 Mouse Monoclonal Antibody [3-G3-D8]
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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Human
概述
产品名称
Cytokeratin 7 Mouse Monoclonal Antibody [3-G3-D8]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within C-terminal human Cytokeratin 7.
种属反应性
Human
验证应用
WB, IHC-P, IF-Cell, IF-Tissue
分子量
Predicted band size: 51 kDa
阳性对照
MCF-7 cell lysate, A549 cell lysate, Hela cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, SKOV-3 cell lysate, SW1990 cell lysate, human lung bronchus tissue, human breast tissue, human lung tissue, human breast carcinoma tissue, human lung carcinoma tissue.
偶联
unconjugated
克隆号
3-G3-D8
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:200-1:800
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IF-Cell
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1:100
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IF-Tissue
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1:1,000
靶点
功能
Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.
背景文献
1. "Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7." Lin L., Holbro T., Alonso G., Gerosa D., Burger M.M. J. Cell. Biochem. 80:483-490(2001)
2. "Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse." Smith F.J.D., Porter R.M., Corden L.D., Lunny D.P., Lane E.B., McLean W.H.I. Biochem. Biophys. Res. Commun. 297:818-827(2002)javascript:;
3. "G protein-coupled estrogen receptor 1/G protein-coupled receptor 30 localizes in the plasma membrane and traffics intracellularly on cytokeratin intermediate filaments." Sanden C., Broselid S., Cornmark L., Andersson K., Daszkiewicz-Nilsson J., Martensson U.E., Olde B., Leeb-Lundberg L.M. Mol. Pharmacol. 79:400-410(2011)
序列相似性
Belongs to the intermediate filament family.
组织特异性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
翻译后修饰
Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
亚细胞定位
Cytoplasm.
UNIPROT
别名
CK 7 antibody
CK-7 antibody
CK7 antibody
Cytokeratin 7 antibody
Cytokeratin-7 antibody
D15Wsu77e antibody
K2C7 antibody
K2C7_HUMAN antibody
K7 antibody
Keratin 7 antibody
展开图片
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☑ Knockout (KO)
All lanes: Western blot analysis of Cytokeratin-7 with anti-Cytokeratin-7 antibody [3-G3-D8] (EM0702) at 1:1,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate.
Lane 3/4: Cytokeratin-7 fragment 1 knockout Hela whole cell lysate.
Lane 5/6: Cytokeratin-7 fragment 2 knockout Hela whole cell lysate.
EM0702 was shown to specifically react with Cytokeratin-7 in wild-type Hela cells. No band was observed when Cytokeratin-7 knockout samples were tested. Wild-type and Cytokeratin-7 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0702, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, ET1705-94, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Cytokeratin 7 on different lysates with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: SK-Br-3 cell lysate
Lane 5: SK-OV-3 cell lysate
Lane 6: SW1990 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0702) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung bronchus tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0702) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of A549 cells labeling Cytokeratin 7 with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 7 antibody (EM0702) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Application: IF-Tissue
Species: Human
Site: lung carcinoma
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"