Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]
Catalog# HA721864
Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]
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WB
-
Human
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Mouse
-
Rat
概述
产品名称
Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C-gamma.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 40 kDa
阳性对照
NIH/3T3 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate.
偶联
unconjugated
克隆号
JE45-17
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
The catalytic subunit α of protein kinase A is a key regulatory enzyme that in humans is encoded by the PRKACA gene. cAMP-dependent protein kinase catalytic subunit beta is an enzyme that in humans is encoded by the PRKACB gene. cAMP-dependent protein kinase catalytic subunit gamma is an enzyme that in humans is encoded by the PRKACG gene. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. Defective regulation of PKA holoenzyme activity has been linked to the progression of cardiovascular disease, certain endocrine disorders and cancers.
背景文献
1. Bauer J et al. The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma. Nat Commun. 2022 Oct
2. Palencia-Campos A et al. Germline and Mosaic Variants in PRKACA and PRKACB Cause a Multiple Congenital Malformation Syndrome. Am J Hum Genet. 2020 Nov
亚细胞定位
Cytoplasm, Cell membrane, Nucleus.
UNIPROT
别名
cAMP dependent protein kinase catalytic subunit alpha antibody
cAMP dependent protein kinase catalytic subunit beta antibody
cAMP dependent protein kinase catalytic subunit gamma antibody
PKA C alpha antibody
PKA C beta antibody
PKA C gamma antibody
PKACA antibody
PRKACA antibody
PRKACB antibody
PRKACG antibody
图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) on different lysates with Rabbit anti-Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) antibody (HA721864) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: C6 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721864) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"