Phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE55-54]
Catalog# HA721516
Phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE55-54]
-
WB
-
Human
-
Mouse
-
Rat
概述
产品名称
Phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE55-54]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 277 kDa
阳性对照
SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes whole cell lysate.
偶联
unconjugated
克隆号
JE55-54
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme (EC 6.4.1.2) that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the cytoplasm of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs —ACACA and ACACB.
背景文献
1. Bates J et al. Acetyl-CoA carboxylase inhibition disrupts metabolic reprogramming during hepatic stellate cell activation. J Hepatol. 2020 Oct
2. Chan GCW et al. Acetyl-coenzyme A carboxylase beta gene polymorphism does not predict cardiovascular risk susceptibility in Chinese type 2 diabetic individuals. Nephrology (Carlton). 2022 May
亚细胞定位
Mitochondrion.
别名
ACAC antibody
ACACA antibody
ACACA_HUMAN antibody
ACC alpha antibody
ACC antibody
ACC-alpha antibody
ACC1 antibody
ACCA antibody
Acetyl CoA carboxylase 1 antibody
Acetyl CoA carboxylase alpha antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-Acetyl Coenzyme A Carboxylase (S79) on different lysates with Rabbit anti-Phospho-Acetyl Coenzyme A Carboxylase (S79) antibody (HA721516) at 1/1,000 dilution.
Lane 1: SH-SY5Y whole cell lysate
Lane 2: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes whole cell lysate
Lane 3: SH-SY5Y treated with λpp for 1 hour whole cell lysate
Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 277 kDa
Observed band size: 277 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE63-95]
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
