SLC40A1 Mouse Monoclonal Antibody [A10A1]
Catalog# HA601178
SLC40A1 Mouse Monoclonal Antibody [A10A1]
-
WB
-
Human
概述
产品名称
SLC40A1 Mouse Monoclonal Antibody [A10A1]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within human SLC40A1 aa 257-306 / 571.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 63 kDa
阳性对照
HEK-293 cell lysate, HepG2 cell lysate, A375 cell lysate, NCCIT cell lysate, Caco-2 cell lysate, SW480 cell lysate.
偶联
unconjugated
克隆号
A10A1
RRID
产品特性
形态
Liquid
浓度
0.87ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Ferroportin-1, also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a protein that in humans is encoded by the SLC40A1 gene, and is part of the Ferroportin (Fpn) Family. Ferroportin is a transmembrane protein that transports iron from the inside of a cell to the outside of the cell. Ferroportin is the only known iron exporter. After dietary iron is absorbed into the cells of the small intestine, ferroportin allows that iron to be transported out of those cells and into the bloodstream. Fpn also mediates the efflux of iron recycled from macrophages resident in the spleen and liver. Ferroportin is regulated by hepcidin, a hormone produced by the liver; hepcidin binds to Fpn and limits its iron-efflux activity, thereby reducing iron delivery to the blood plasma. Therefore, the interaction between Fpn and hepcidin controls systemic iron homeostasis.
背景文献
1. Hao L et al. SLC40A1 Mediates Ferroptosis and Cognitive Dysfunction in Type 1 Diabetes. Neuroscience. 2021 May
2. Huang B et al. Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization. Oxid Med Cell Longev. 2022 Aug
亚细胞定位
Cell membrane, Basolateral cell membrane.
UNIPROT #
别名
Ferroportin 1 antibody
Ferroportin antibody
Ferroportin-1 antibody
FPN1 antibody
HFE4 antibody
IREG1 antibody
iron regulated gene 1 antibody
Iron regulated transporter 1 antibody
Iron-regulated transporter 1 antibody
MST079 antibody
展开图片
-
Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (HA601178) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A375 cell lysate
Lane 4: NCCIT cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (HA601178) at 1/1,000 dilution.
Lane 1: Caco-2 cell lysate
Lane 2: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
