Skip to content

CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]

RMB: 1500.00

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# HA721013

CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]

Application

  • WB

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human CD100 aa 126-175/862.

种属反应性

Human

验证应用

WB

分子量

Predicted band size: 96 kDa

阳性对照

Jurkat cell lysate, Daudi cell lysate, HL-60 cell lysate.

偶联

unconjugated

克隆号

JE63-21

RRID

AB_3072137

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

发表文章中的应用

WB See 1 publications below
IF See 1 publications below

发表文章中的种属

Human See 1 publications below
Mouse See 1 publications below

靶点

功能

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.

背景文献

1. Wang L. et. al. The emerging roles of semaphorin4D/CD100 in immunological diseases. Biochem Soc Trans. 2020 Dec

2. Kalina T. et. al. CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets. Front Immunol. 2019 Oct

亚细胞定位

Cell membrane.

UNIPROT #

SwissProt: Q92854 Human

别名

A8 antibody

BB 18 antibody

BB18 antibody

CD 100 antibody

CD100 antibody

CD100 antigen antibody

Coll 4 antibody

COLL4 antibody

Collapsin 4 antibody

Collapsin4 antibody

展开

图片

  • Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/500 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: Daudi cell lysate<br />Lane 3: HL-60 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 30 seconds;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/500 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: HL-60 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 130 kDa

    Exposure time: 30 seconds;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/2,000 dilution. Lane 1: HAP1-si NT cell lysate Lane 2: HAP1-si CD100 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 62 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/2,000 dilution. Lane 1: HAP1-si NT cell lysate Lane 2: HAP1-si CD100 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 62 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IF

同靶点&同通路的产品

metafield image

CD100 Mouse Monoclonal Antibody [5H6E3]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单