• 概述

• 产品描述 C-C chemokine receptor type 7 is a protein that in humans is encoded by the CCR7 gene. Two ligands have been identified for this receptor: the chemokines (C-C motif) ligand 19 (CCL19/ELC) and (C-C motif) ligand 21 (CCL21). CCR7 has also recently been designated CD197 (cluster of differentiation 197). The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein–Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. CCR7 has been shown to stimulate dendritic cell maturation. CCR7 is also involved in homing of T cells to various secondary lymphoid organs such as lymph nodes and the spleen as well as trafficking of T cells within the spleen. Activation of dendritic cells in peripheral tissues induces CCR7 expression on the cell's surface, which recognize CCL19 and CCL21 produced in the Lymph node and increases dendritic cell expression of co-stimulation molecules (B7), and MHC class I or MHC class II.
• 产品名称 iFluor™ 488 Conjugated Anti-CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04]
• 分子量 Predicted band size: 43 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 IF-Cell,IF-Tissue
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human CCR7 aa 13-62 / 378.
• 偶联 iFluor™ 488

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cell membrane.
• 数据链接 SwissProt: P32248 Human
  Unigene: 229736 Rat
  Entrez Gene: 287673 Rat
  
• 其它名称
  • BLR 2 antibody
  • BLR2 antibody
  • C C chemokine receptor type 7 antibody
  • C C CKR 7 antibody
  • C-C chemokine receptor type 7 antibody
  • C-C CKR-7 antibody
  • CC chemokine receptor 7 antibody
  • CC chemokine receptor type 7 antibody
  • CC CKR 7 antibody
  • CC-CKR-7 antibody
  • CCCKR7 antibody
  • CCR 7 antibody
  • CCR-7 antibody
  • Ccr7 antibody
  • CCR7_HUMAN antibody
  • CD 197 antibody
  • CD197 antibody
  • CD197 antigen antibody
  • CDw197 antibody
  • Chemokine (C C motif) receptor 7 antibody
  • Chemokine (C C) receptor 7 antibody
  • Chemokine C C motif receptor 7 antibody
  • Chemokine C C receptor 7 antibody
  • Chemokine receptor 7 antibody
  • Chemokine receptor 7-like protein antibody
  • CMKBR7 antibody
  • EBI 1 antibody
  • EBI1 antibody
  • Ebi1h antibody
  • EBV Induced G Protein Coupled Receptor 1 antibody
  • EBV-induced G-protein coupled receptor 1 antibody
  • Epstein Barr virus induced G protein coupled receptor antibody
  • Epstein Barr virus induced gene 1 antibody
  • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
  • EVI 1 antibody
  • EVI1 antibody
  • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
  • MGC108519 antibody
  • MIP 3 beta receptor antibody
  • MIP-3 beta receptor antibody
  • MIP3 Beta Receptor antibody
  • iFluor™488ConjugatedAnti-CCR7
  more

• 应用

  IF-Cell: 1:100
  IF-Tissue: 1:50

• 

  Fig1: Immunocytochemistry analysis of MCF-7 cells labeling CCR7 with Rabbit anti-CCR7 antibody (HA720157F) at 1/100 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-CCR7 antibody (HA720157F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  

  Fig2: Immunofluorescence analysis of paraffin-embedded rat spleen tissue labeling CCR7 (HA720157F).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody CCR7 (HA720157F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  

  Fig3: Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling CCR7 (HA720157F).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody CCR7 (HA720157F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

• 背景文献

• 1. Pang MF et al. TGF-β1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Oncogene N/A:N/A (2015).
  
  2. Guo J et al. Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncol Lett 5:1572-1578 (2013).

• Publishing research using iFluor™ 488 Conjugated Anti-CCR7 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.