Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium.
Goat anti-Mouse IgG-FITC antibody
Mouse IgG (H+L).
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.5%BSA, 40%Glycerol.
Protein affinity purified.
Immunohistochemistry: 1:100 – 1:1,000
Immunocytochemistry: 1:100 – 1:1,000
Publishing research using Goat anti-Mouse IgG-FITC antibody? Please let us know so
that we can cite the reference in this datasheet.
He, L., Hu, X., Zhu, M., Liang, Z., Chen, F., Zhu, L., . . . Gong, C. (2017). Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus. Gene, 627, 343-350.
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