您的当前位置: 首页 > 产品 >产品详情

Goat anti-Rabbit IgG-HRP antibody

羊多抗 | 货号: HA1001


规格:
价格:RMB   200
  • 概述

  • 产品描述 Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
  • 产品名称 Goat anti-Rabbit IgG-HRP antibody
  • 种属反应性 Rabbit
  • 验证应用 WB, ELISA, IHC
  • 抗体类型 羊多抗
  • 免疫原 Rabbit IgG (H+L).
  • 偶联 HRP-conjugated
  • 性能

  • 形态 Liquid
  • 浓度 1 mg/mL.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol.
  • 亚型 IgG
  • 纯化方式 Protein affinity purified.
  • 应用

    WB: 1:10,000~1:50,000
    ELISA: 1:5,000~1:10,000

  • 背景文献

  • 暂无
  • Publishing research using Goat anti-Rabbit IgG-HRP antibody? Please let us know so that we can cite the reference in this datasheet.

  • Gu, B., Zhang, J., Wang, W., Mo, L., Zhou, Y., Chen, L., . . . Zhang, M. (2010). Global expression of cell surface proteins in embryonic stem cells. PLoS One, 5(12), e15795. PMID:21209962
  • Xie, Q. R., Liu, Y., Shao, J., Yang, J., Liu, T., Zhang, T., . . . Xia, W. (2013). Male contraceptive Adjudin is a potential anti-cancer drug. Biochem Pharmacol, 85(3), 345-355. PMID:23178657
  • Sun, K., Xie, X., Liu, Y., Han, Z., Zhao, X., Cai, N., . . . Wei, L. (2013). Autophagy lessens ischemic liver injury by reducing oxidative damage. Cell Biosci, 3(1), 26 PMID:23758862
  • Hu, X., Yang, R., Zhang, X., Chen, L., Xiang, X., Gong, C., & Wu, X. (2013). Molecular cloning and functional characterization of the dual oxidase (BmDuox) gene from the silkworm Bombyx mori. PLoS One, 8(8), e70118. PMID:23936382
  • Xue, X. B., Zou, L. F., Ma, W. X., Liu, Z. Y., & Chen, G. Y. (2014). Identification of 17 HrpX-regulated proteins including two novel type III effectors, XOC_3956 and XOC_1550, in Xanthomonas oryzae pv. oryzicola. PLoS One, 9(3), e93205 PMID:24675748
  • He, X. J., Tao, H. Q., Hu, Z. M., Ma, Y. Y., Xu, J., Wang, H. J., . . . Chen, J. Z. (2014). Expression of galectin-1 in carcinoma-associated fibroblasts promotes gastric cancer cell invasion through upregulation of integrin beta1. Cancer Sci, 105(11), 140 PMID:25230369
  • Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y., . . . Li, Z. (2015). Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress-associated apoptosis in HeLa cells. Mol Med Rep, 11(1), 189-195. PMID:25323748
  • Chen, Q. H., Liu, A. R., Qiu, H. B., & Yang, Y. (2015). Interaction between mesenchymal stem cells and endothelial cells restores endothelial permeability via paracrine hepatocyte growth factor in vitro. Stem Cell Res Ther, 6, 44. PMID:25888925
  • Sun, X. Y., Qin, H. J., Zhang, Z., Xu, Y., Yang, X. C., Zhao, D. M., . . . Sun, L. K. (2016). Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stressinduced apoptosis. Mol Med Rep, 13(1), 661-668. PMID:26647757
  • Yang, Y., Chen, Q. H., Liu, A. R., Xu, X. P., Han, J. B., & Qiu, H. B. (2015). Synergism of MSC-secreted HGF and VEGF in stabilising endothelial barrier function upon lipopolysaccharide stimulation via the Rac1 pathway. Stem Cell Res Ther, 6, 250. PMID:26674641
  • Lu, Y., Zhang, T. F., Shi, Y., Zhou, H. W., Chen, Q., Wei, B. Y., . . . Fu, C. Y. (2016). PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells. Sci Rep, 6, 20823. PMID:26860588
  • Gao, F., Gu, Q. J., Pan, J., Wang, Z. H., Yin, C. L., Li, F., . . . Shi, M. (2016). Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism. Sci Rep, 6, 26967. PMID:27254821
  • Wu, Y., Qian, Y., Zhou, G., Lv, J., Yan, Q., & Dong, X. (2016). Effect of GEN1 interference on the chemosensitivity of the breast cancer MCF-7 and SKBR3 cell lines. Oncol Lett, 11(6), 3597-3604. PMID:27284361

Choose your product,application and species below,then click "Submit".

On the next page you can complete and submit your review.

  • * Code No.
  • *Application
  • *Sample
  • Species Tested
  • Against
  • Choose text
  • Content

Leave us a review

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols