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Anti-MSH2 antibody [10G1]

小鼠单抗 | 货号: EM1801-04


规格:
价格:RMB   2500
  • 概述

  • 产品描述 Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
  • 产品名称 Anti-MSH2 antibody [10G1]
  • 分子量 104 kDa
  • 种属反应性 Human,Rat
  • 验证应用 WB,IHC
  • 抗体类型 小鼠单抗
  • 免疫原 Synthetic peptide within N-terminal residues of Human MSH2.
  • 偶联 Non-conjugated
  • 性能

  • 形态 Liquid
  • 浓度 2 mg/mL.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
  • 亚型 IgG2b
  • 纯化方式 ProG purified.
  • 亚细胞定位 Nucleus, Chromosome.
  • 数据链接 SwissProt: P43246 Human
    SwissProt: P54275 Rat
  • 其它名称 BAT26 antibody
    COCA 1 antibody
    COCA1 antibody
    DNA mismatch repair protein Msh2 antibody
    FCC 1 antibody
    FCC1 antibody
    hMSH2 antibody
    HNPCC 1 antibody
    HNPCC antibody
    HNPCC1 antibody
    LCFS2 antibody
    MSH 2 antibody
    Msh2 antibody
    MSH2_HUMAN antibody
    MutS homolog 2 antibody
    MutS homolog 2 colon cancer nonpolyposis type 1 antibody
    MutS protein homolog 2 antibody
  • 应用

    WB: 1:2,000-1:5,000
    IHC: 1:100-1:500

  • 背景文献

  • 1. Kansikas M. et al. Verification of the three-step model in assessing the pathogenicity of mismatch repair gene variants. Hum. Mutat. 32:107-115(2011).
    2. Traver S. et al. MCM9 Is Required for Mammalian DNA Mismatch Repair. Mol. Cell 59:831-839(2015).
  • Publishing research using Anti-MSH2 antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols