MUC1 Mouse Monoclonal Antibody [PSH0-49]
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概述
产品名称
MUC1 Mouse Monoclonal Antibody [PSH0-49]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide of core peptide domain of human MUC1.
种属反应性
Human
验证应用
IHC-P, FC, IF-Cell
分子量
Predicted band size: 122 kDa
阳性对照
Human breast carcinoma tissue, human kidney tissue, human lung tissue, human tonsil tissue, HeLa, SK-Br-3.
偶联
unconjugated
克隆号
PSH0-49
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2a
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
-
1:5,000-1:10,000
-
FC
-
1:500-1:1,000
-
IF-Cell
-
1:500
靶点
功能
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.
背景文献
1. Taylor-Papadimitriou J. et. al. Latest developments in MUC1 immunotherapy. Biochem Soc Trans. 2018 Jun
2. Kato K. et. al. MUC1: The First Respiratory Mucin with an Anti-Inflammatory Function. J Clin Med. 2017 Nov
组织特异性
Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
翻译后修饰
Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.; Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.; Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.; Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.; The N-terminal sequence has been shown to begin at position 24 or 28.
亚细胞定位
Apical cell membrane. Secreted. Nucleus, Cell membrane, Cytoplasm.
UNIPROT #
别名
ADMCKD antibody
ADMCKD1 antibody
Breast carcinoma associated antigen DF3 antibody
Breast carcinoma-associated antigen DF3 antibody
CA 15-3 antibody
CA15 3 antibody
CA15 3 antigen antibody
CA15-3 antibody
CA15.3 antibody
Cancer antigen 15-3 antibody
展开ADMCKD antibody
ADMCKD1 antibody
Breast carcinoma associated antigen DF3 antibody
Breast carcinoma-associated antigen DF3 antibody
CA 15-3 antibody
CA15 3 antibody
CA15 3 antigen antibody
CA15-3 antibody
CA15.3 antibody
Cancer antigen 15-3 antibody
Carcinoma associated mucin antibody
Carcinoma-associated mucin antibody
CD 227 antibody
CD227 antibody
DF3 antigen antibody
EMA antibody
Episialin antibody
Epithelial Membrane Antigen antibody
H23 antigen antibody
H23AG antibody
KL 6 antibody
KL-6 antibody
KL6 antibody
Krebs von den Lungen-6 antibody
MAM 6 antibody
MAM6 antibody
MCD antibody
MCKD antibody
MCKD1 antibody
Medullary cystic kidney disease 1 (autosomal dominant) antibody
Medullary cystic kidney disease, autosomal dominant antibody
MUC 1 antibody
MUC-1 antibody
MUC-1/SEC antibody
MUC-1/X antibody
MUC1 antibody
MUC1-alpha antibody
MUC1-beta antibody
MUC1-CT antibody
MUC1-NT antibody
MUC1/ZD antibody
MUC1_HUMAN antibody
Mucin 1 antibody
Mucin 1 cell surface associated antibody
Mucin 1 transmembrane antibody
Mucin 1, cell surface associated antibody
Mucin-1 subunit beta antibody
Peanut reactive urinary mucin antibody
Peanut-reactive urinary mucin antibody
PEM antibody
PEMT antibody
Polymorphic epithelial mucin antibody
PUM antibody
Tumor associated epithelial membrane antigen antibody
Tumor associated epithelial mucin antibody
Tumor associated mucin antibody
Tumor-associated epithelial membrane antigen antibody
Tumor-associated mucin antibody
折叠图片
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MUC1 antibody (HA601142) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601142) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-MUC1 antibody (HA601142) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601142) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-MUC1 antibody (HA601142) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601142) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MUC1 antibody (HA601142) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601142) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) labeling MUC1 with Mouse anti-MUC1 antibody (HA601142) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MUC1 antibody (HA601142) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of SK-Br-3 cells labeling MUC1.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601142, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of HeLa cells labeling MUC1.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601142, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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