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iFluor™ 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH monoclonal Antibody

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Catalog# HA1129

iFluor™ 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH monoclonal Antibody

Application

  • IF-Cell

  • IHC-Fr

Reactivity

  • Rabbit

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

iFluor™ 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH monoclonal Antibody

抗体类型

Alpaca Monoclonal Antibody

免疫原

Fc region of Rabbit IgG.

种属反应性

Rabbit

验证应用

IF-Cell, IHC-Fr

偶联

iFluor™ 488

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • IF-Cell

  • 1:500-1:1,000

  • IHC-Fr

  • 1:500-1:1,000

发表文章中的应用

IF See 1 publications below

靶点

功能

Higher resolution imaging due to the Alpaca anti-rabbit IgG Fc, recombinant VHHs’ 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.

背景文献

暂无

图片

  • Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (<a href="/products/HA721174" style="font-weight: bold;text-decoration: underline;">HA721174</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (<a href="/products/HA721174" style="font-weight: bold;text-decoration: underline;">HA721174</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor&trade; 488, <a href="/products/HA1129" style="font-weight: bold;text-decoration: underline;">HA1129</a>) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA721174) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA721174) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor™ 488, HA1129) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of HeLa cells labeling Skp2 with Rabbit anti-Skp2 antibody (<a href="/products/HA500427" style="font-weight: bold;text-decoration: underline;">HA500427</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Skp2 antibody (<a href="/products/HA500427" style="font-weight: bold;text-decoration: underline;">HA500427</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor&trade; 488, <a href="/products/HA1129" style="font-weight: bold;text-decoration: underline;">HA1129</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling Skp2 with Rabbit anti-Skp2 antibody (HA500427) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Skp2 antibody (HA500427) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor™ 488, HA1129) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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