概述
产品名称
SOD2 Recombinant Rabbit Monoclonal Antibody [JJ089-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human SOD2 aa 1-50 / 222.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 25 kDa
阳性对照
Mouse brain tissue lysate, SH-SY5Y cell lysate, mouse heart tissue lysate, human liver tissue, human kidney tissue, mouse liver tissue, mouse colon tissue, mouse heart tissue.
偶联
unconjugated
克隆号
JJ089-02
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:50-1:200
发表文章中的应用
靶点
功能
The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer’s disease, Parkinson’s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans.
背景文献
1. Carlson RI et al. Development and application of an antibody-based protein microarray to assess physiological stress in grizzly bears (Ursus arctos). Conserv Physiol 4:cow001 (2016).
2. Greco T et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016).
序列相似性
Belongs to the iron/manganese superoxide dismutase family.
翻译后修饰
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.; Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity).; Polyubiquitinated; leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.
亚细胞定位
Mitochondrion matrix.
UNIPROT #
别名
Indophenoloxidase B antibody
IPO B antibody
IPOB antibody
Manganese containing superoxide dismutase antibody
Manganese SOD antibody
Manganese superoxide dismutase antibody
Mangano superoxide dismutase antibody
Mn SOD antibody
Mn superoxide dismutase antibody
MNSOD antibody
展开Indophenoloxidase B antibody
IPO B antibody
IPOB antibody
Manganese containing superoxide dismutase antibody
Manganese SOD antibody
Manganese superoxide dismutase antibody
Mangano superoxide dismutase antibody
Mn SOD antibody
Mn superoxide dismutase antibody
MNSOD antibody
MVCD6 antibody
SOD 2 antibody
SOD2 antibody
SODM_HUMAN antibody
Superoxide dismutase [Mn] mitochondrial antibody
Superoxide dismutase [Mn], mitochondrial antibody
Superoxide dismutase 2 mitochondrial antibody
折叠图片
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Western blot analysis of SOD2 on different lysates with Rabbit anti-SOD2 antibody (ET1701-54) at 1/500 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: SH-SY5Y cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-54) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Western blot analysis of SOD2 on different lysates with Rabbit anti-SOD2 antibody (ET1701-54) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: Human liver tissue lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Rat liver tissue lysate
Cell lysates/proteins at 20 µg/Lane.
Tissue lysates/proteins at 40 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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CLPs-miR-103a-2-5p inhibits proliferation and promotes cell apoptosis in AML cells by targeting LILRB3 and Nrf2/HO-1 axis, regulating CD8 + T cell response
Author: Cen Qingyan,et al
PMID: 38486250
应用: WB,IHC
反应种属: Human
发表时间: 2024 Mar
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Citation
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The Tanshinones (Tan) Extract From Salvia miltiorrhiza Bunge Induces ROS-Dependent Apoptosis in Pancreatic Cancer via AKT Hyperactivation-Mediated FOXO3/SOD2 Signaling
Author: Xu Qin,et al
PMID: 38899834
应用: WB
反应种属: Human
发表时间: 2024 Jun
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Citation
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Low-level Nd:YAG laser inhibiting inflammation and oxidative stress in human gingival fibroblasts via AMPK/SIRT3 axis
Author: Zhang Haizheng,et al
PMID: 38244301
应用: WB,IF
反应种属: Human
发表时间: 2024 Jan
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Citation
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Costunolide ameliorates MNNG-induced chronic atrophic gastritis through inhibiting oxidative stress and DNA damage via activation of Nrf2
Author: Wang Ruixuan,et al
PMID: no pmid0506
应用: WB
反应种属: Human
发表时间: 2024 Apr
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Citation
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PD184352 exerts anti-inflammatory and antioxidant effects by promoting activation of the Nrf2/HO-1 axis
Author:
PMID: 37028460
应用: WB
反应种属: Mouse
发表时间: 2023 May
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Citation
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Nuclear factor-Y mediates pancreatic β-cell compensation by repressing reactive oxygen species-induced apoptosis under metabolic stress
Author: He, S., Yu, X., Cui, D., Liu, Y., Yang, S., Zhang, H., Hu, W., & Su, Z.
PMID: 37000974
应用: WB
反应种属: Mouse
发表时间: 2023 Apr
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Citation
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Fraxinellone inhibits progression of glioblastoma via regulating the SIRT3 signaling pathway
Author: Shi, J., Sun, S., Xing, S., Huang, C., Huang, Y., Wang, Q., Xue, X., Chen, Z., Wang, Y., & Huang, Z.
PMID: 36076484
应用: WB
反应种属: Rat
发表时间: 2022 Sept
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Citation
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SIRT3-Mediated CypD-K166 Deacetylation Alleviates Neuropathic Pain by Improving Mitochondrial Dysfunction and Inhibiting Oxidative Stress
Author: Yan, B., Liu, Q., Ding, X., Lin, Y., Jiao, X., Wu, Y., Miao, H., & Zhou, C.
PMID: 36092157
应用: WB
反应种属: Mouse
发表时间: 2022 Sept
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Citation
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Cholesterol Sulfate Exerts Protective Effect on Pancreatic β-Cells by Regulating β-Cell Mass and Insulin Secretion
Author: Zhang, X., Deng, D., Cui, D., Liu, Y., He, S., Zhang, H., Xie, Y., Yu, X., Yang, S., Chen, Y., & Su, Z.
PMID: 35308228
应用: WB
反应种属: Mouse
发表时间: 2022 Mar
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Citation
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Cdk5-mediated phosphorylation of Sirt1 contributes to podocyte mitochondrial dysfunction in diabetic nephropathy
Author:
PMID: 32660255
应用: WB
反应种属: Mouse,Human
发表时间: 2021 Jan
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Citation
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Iron Overload Protects from Obesity by Ferroptosis
Author: Ma, W., Jia, L., Xiong, Q., & Du, H.
PMID: 34441564
应用: WB
反应种属: Mouse
发表时间: 2021 Aug
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Citation
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Effect of resveratrol on mouse ovarian vitrification and transplantation. Reproductive biology and endocrinology : RB&E, 19(1), 54.
Author: Wang, D., Geng, M., Gan, D., Han, G., Gao, G., Xing, A., Cui, Y., & Hu, Y.
PMID: 33836793
应用: WB,IHC
反应种属: Mouse
发表时间: 2021 Apr
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Citation
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Curcumin attenuates MSU crystal-induced inflammation by inhibiting the degradation of IκBα and blocking mitochondrial damage.
Author:
PMID: 31455356
应用: WB
反应种属: Human
发表时间: 2019 Aug
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Citation