iFluor™ 647 Conjugated Ki67 Recombinant Rabbit Monoclonal Antibody [SR00-02]

Immunocytochemistry analysis of A431 cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA720163F) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67 antibody (HA720163F, red) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

Immunofluorescence analysis of paraffin-embedded Jurkat cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA720163F) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720163F, red) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Ki67 (HA720163F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Ki67 (HA720163F, red) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of frozen mouse small intestine tissue with Rabbit anti-Ki67 antibody (HA720163F) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720163F, red ) at 1/500 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

Flow cytometric analysis of MOLT-4 cells labeling Ki67.

Cells were washed twice in 1x PBS and fixed in 70% ethanol at 4℃ overnight. Then incubated for 1 hour at +4℃ with Ki67 (HA720163F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).