HIF-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH07-00]
Catalog# HA722778
HIF-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH07-00]
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WB
-
IF-Cell
-
IHC-P
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Human
-
Mouse
概述
产品名称
HIF-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH07-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human HIF-1 alpha aa 441-490.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 93 kDa
阳性对照
HeLa cell lysate, HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate, HepG2 cell lysate, HepG2 treated with 100μM CoCl2 for 4 hours cell lysate, C2C12 cell lysate, C2C12 treated with 100μM CoCl2 for 4 hours cell lysate, HeLa cells treated with 0.5mM CoCl2 for 6 hours, C2C12 cells treated with 200μM CoCl2 for 4 hours.
偶联
unconjugated
克隆号
PSH07-00
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IF-Cell
-
1:100
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IHC-P
-
1:1,000
靶点
功能
Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a subunit of a heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) that is encoded by the HIF1A gene. The Nobel Prize in Physiology or Medicine 2019 was awarded for the discovery of HIF. HIF1A is a basic helix-loop-helix PAS domain containing protein, and is considered as the master transcriptional regulator of cellular and developmental response to hypoxia. The dysregulation and overexpression of HIF1A by either hypoxia or genetic alternations have been heavily implicated in cancer biology, as well as a number of other pathophysiologies, specifically in areas of vascularization and angiogenesis, energy metabolism, cell survival, and tumor invasion. Two other alternative transcripts encoding different isoforms have been identified.
背景文献
1. Korbecki J et al. Chronic and Cycling Hypoxia: Drivers of Cancer Chronic Inflammation through HIF-1 and NF-κB Activation: A Review of the Molecular Mechanisms. Int J Mol Sci. 2021 Oct
2. Infantino V et al. Cancer Cell Metabolism in Hypoxia: Role of HIF-1 as Key Regulator and Therapeutic Target. Int J Mol Sci. 2021 May
亚细胞定位
Cytoplasm, Nucleus, Nucleus speckle.
别名
ARNT interacting protein antibody
ARNT-interacting protein antibody
Basic helix loop helix PAS protein MOP1 antibody
Basic-helix-loop-helix-PAS protein MOP1 antibody
bHLHe78 antibody
Class E basic helix-loop-helix protein 78 antibody
HIF 1A antibody
HIF 1alpha antibody
HIF-1-alpha antibody
HIF-1alpha antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA722778) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HepG2 treated with 100μM CoCl2 for 4 hours cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 100μM CoCl2 for 4 hours cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722778) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells treated with 0.5mM CoCl2 for 6 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (HA722778) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (HA722778) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of C2C12 cells treated with 200μM CoCl2 for 4 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (HA722778) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (HA722778) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded HeLa cells treated with 0.5mM CoCl2 for 6 hours with Rabbit anti-HIF-1 alpha antibody (HA722778) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722778) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
HIF-1 alpha Mouse Monoclonal Antibody [5H7]
Application: WB,IP,ICC,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
