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Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

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Catalog# ET1705-55

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

Application

  • IHC-P

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within C-terminal human Ferritin Heavy Chain.

种属反应性

Human

验证应用

IHC-P

分子量

Predicted band size: 21 kDa

阳性对照

Human kidney tissue, human liver tissue, human placenta tissue.

偶联

unconjugated

克隆号

JM22-36

RRID

AB_3070583

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-P

  • 1:200-1:1,000

发表文章中的应用

WB See 3 publications below

发表文章中的种属

Human See 2 publications below
Mouse See 1 publications below

靶点

功能

Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.

背景文献

1. Vannucci L et al. In Vivo Targeting of Cutaneous Melanoma Using an Melanoma Stimulating Hormone-Engineered Human Protein Cage with Fluorophore and Magnetic Resonance Imaging Tracers. J Biomed Nanotechnol 11:81-92 (2015).

2. Yang H et al. Upregulation of mitochondrial ferritin by proinflammatory cytokines: implications for a role in Alzheimer\'s disease. J Alzheimers Dis 45:797-811 (2015).

序列相似性

Belongs to the ferritin family.

组织特异性

Expressed in the liver.

亚细胞定位

Cytoplasm. Nucleus.

UNIPROT #

SwissProt: P02794 Human

别名

Apoferritin antibody

Cell proliferation inducing gene 15 protein antibody

Cell proliferation-inducing gene 15 protein antibody

F HC antibody

Ferritin H subunit antibody

Ferritin heavy chain antibody

Ferritin heavy polypeptide 1 antibody

FHC antibody

FRIH antibody

FRIH_HUMAN antibody

展开

图片

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1705-55) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1705-55) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

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Conjugate: unconjugated

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