ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]
Catalog# ET1703-60
ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
概述
产品名称
ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human ADAM10 aa 715-748 / 748.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP
分子量
Predicted band size: 84 kDa
阳性对照
Jurkat cell lysate, HeLa cell lysate, human lung tissue lysate, RAW264.7 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat spleen tissue lysate, human thyroid carcinoma tissue, human stomach tissue.
偶联
unconjugated
克隆号
JM32-11
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:1,000
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IP
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1-2μg/sample
发表文章中的应用
| Co-IP | See 1 publications below |
发表文章中的种属
| Mouse | See 1 publications below |
靶点
功能
ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNFα (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (integrin ligand) a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM10 is a TNF-processing enzyme that cleaves pro-TNF, a membrane-bound precusor protein, at Ala 76-Val 77, which causes membrane shedding of soluble TNF.
背景文献
1. Yang C et al. Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer transgenic mouse model. Neuropeptides 48:233-8 (2014).
2. Guo Q et al. Adam10 Mediates the Choice between Principal Cells and Intercalated Cells in the Kidney. J Am Soc Nephrol N/A:N/A (2014).
组织特异性
Expressed in the brain (at protein level). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
翻译后修饰
The precursor is cleaved by furin and PCSK7.
亚细胞定位
Cell membrane. Endomembrane system.
别名
A disintegrin and metalloprotease domain 10 antibody
A disintegrin and metalloproteinase domain 10 antibody
AD 10 antibody
AD10 antibody
AD18 antibody
ADA10_HUMAN antibody
ADAM 10 antibody
ADAM metallopeptidase domain 10 antibody
ADAM10 antibody
CD 156c antibody
展开图片
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Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: Human lung tissue lysate (30 µg/Lane)
Lane 4: RAW264.7 cell lysate (15 µg/Lane)
Lane 5: Mouse placenta tissue lysate (30 µg/Lane)
Lane 6: Mouse brain tissue lysate (30 µg/Lane)
Lane 7: Rat brain tissue lysate (30 µg/Lane)
Lane 8: Rat spleen tissue lysate (30 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 75/100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si ADAM10 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84/70 kDa
Exposure time: 2 minutes ; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-60) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
ADAM10 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1703-60 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-60 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: ET1703-60 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of ET1703-60 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 59 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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ADAM10-mediated β-klotho degradation: a key to FGF21 resistance in senescent vascular endothelial cells
Author: Yuzhen Huang,et al
PMID: 39653972
应用: Co-IP
反应种属: Mouse
发表时间: 2024 Dec
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Citation
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