All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
HRP Conjugated Anti-beta Actin Recombinant Rabbit Monoclonal Antibody
Predicted band size: 42 kDa
Recombinant full length protein of Human beta Actin aa 1-375 / 375.
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.
Protein A affinity purified.
SwissProt: P60709 Human
SwissProt: P60710 Mouse
SwissProt: P60711 Rat
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
- ACTB_HUMAN antibody
- Actin beta antibody
- Actin cytoplasmic 1 antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
- Actx antibody
- b actin antibody
- Beta cytoskeletal actin antibody
- Beta-actin antibody
- BRWS1 antibody
- E430023M04Rik antibody
- MGC128179 antibody
- PS1TP5 binding protein 1 antibody
- PS1TP5BP1 antibody
Fig1: Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (ET1702-67) at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lane 8: Mouse lung tissue lysate
Lane 9: Rat lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-67) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Fig2: Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.
1. Wang H et al. Role of the orexin (hypocretin) system in contextual fear conditioning in rats. Behav Brain Res 316:47-53 (2017).
2. Gruber R et al. YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK-STAT3 Signaling. Gastroenterology 151:526-39 (2016).
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