• 概述

• 产品描述 All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
• 产品名称 HRP Conjugated Anti-beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]
• 分子量 Predicted band size: 42 kDa
• 种属反应性 Human,Mouse,Rat,Zebrafish, Monkey,Hamster,Plant
• 验证应用 WB
• 抗体类型 重组兔单抗
• 免疫原 Recombinant full length protein of Human beta Actin aa 1-375 / 375.
• 偶联 HRP-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm.
• 数据链接 SwissProt: P60709 Human
  SwissProt: P60710 Mouse
  SwissProt: P60711 Rat
  
• 其它名称
  • A26C1A antibody
  • A26C1B antibody
  • ACTB antibody
  • ACTB_HUMAN antibody
  • Actin beta antibody
  • Actin cytoplasmic 1 antibody
  • Actin, cytoplasmic 1, N-terminally processed antibody
  • Actx antibody
  • b actin antibody
  • Beta cytoskeletal actin antibody
  • Beta-actin antibody
  • BRWS1 antibody
  • E430023M04Rik antibody
  • MGC128179 antibody
  • PS1TP5 binding protein 1 antibody
  • PS1TP5BP1 antibody
  • HRPConjugatedAnti-betaActin
  more

• 应用

  WB: 1:10,000

• 

  Fig1: Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (ET1702-67) at 1/10,000 dilution.
  
  Lane 1: HeLa cell lysate
  Lane 2: A549 cell lysate
  Lane 3: Jurkat cell lysate
  Lane 4: NIH/3T3 cell lysate
  Lane 5: PC-12 cell lysate
  Lane 6: Mouse brain tissue lysate
  Lane 7: Rat brain tissue lysate
  Lane 8: Mouse lung tissue lysate
  Lane 9: Rat lung tissue lysate
  
  Lysates/proteins at 20 µg/Lane.
  
  Predicted band size: 42 kDa
  Observed band size: 42 kDa
  
  Exposure time: 30 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-67) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  

  Fig2: Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.

• 背景文献

• 1. Wang H et al. Role of the orexin (hypocretin) system in contextual fear conditioning in rats. Behav Brain Res 316:47-53 (2017).
  
  2. Gruber R et al. YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK-STAT3 Signaling. Gastroenterology 151:526-39 (2016).

• Publishing research using HRP Conjugated Anti-beta Actin Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  37027476 Modification of maternally defined H3K4me3 regulates the inviability of interspecific Xenopus hybrids Author: Long, Q., Yan, K., Wang, C., Wen, Y., Qi, F., Wang, H., Shi, P., Liu, X., Chan, W. Y., Lu, X., & Zhao, H. Published: 2023 Application: WB Species: Xenopus Journal: Sci Adv IF: 14.957
• PMID:
  36437860 Dietary Nε-(carboxymethyl) lysine affects cardiac glucose metabolism and myocardial remodeling in mice Author: Wang, Z. Q., & Sun, Z. Published: 2022 Application: WB Species: Mouse Journal: World J Diabetes IF: 4.560
• PMID:
  33576445 lncRNA‑MALAT1 promotes high glucose‑induced H9C2 cardiomyocyte pyroptosis by downregulating miR‑141‑3p expression. Molecular medicine reports, 23(4), 259. Author: Wu, A., Sun, W., & Mou, F. Published: 2021 Application: WB Species: Rat Journal: Mol Med Rep
• PMID:
  32559839 Ling-gui-zhu-gan decoction alleviates hepatic steatosis through SOCS2 modification by N6-methyladenosine Author: Guang Ji Published: 2020 Application: WB Species: rat Journal: Biomedicine & Pharmacotherapy IF: 4.545
• PMID:
  32628818 Comprehensive analysis of 5‐hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer Author: Guang Ji Published: 2020 Application: WB Species: human Journal: Clin Transl Med. IF: 2.737
• PMID:
  30818128 Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells Author: Yafei Wu Published: 2019 Application: WB Species: HUVEC cell Journal: Arch Oral Biol. IF: 1.663
• PMID:
  30380511 Protective effects of hydrogen gas against sepsis-induced acute lung injury via regulation of mitochondrial function and dynamics. Author: Keliang Xie Published: 2018 Application: WB Species: Mouse Journal: Int Immunopharmacol. IF: 3.118

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.