iFluor™ 594 Conjugated Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody [ST04-85]
Catalog# HA720122F
iFluor™ 594 Conjugated Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody [ST04-85]
-
IF-Tissue
-
Human
-
Mouse
-
Rat
概述
产品名称
iFluor™ 594 Conjugated Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody [ST04-85]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Cytokeratin 15 aa 410-455.
种属反应性
Human, Mouse, Rat
验证应用
IF-Tissue
分子量
49 kDa
阳性对照
Human skin tissue, rat skin tissue.
偶联
iFluor™ 594
克隆号
ST04-85
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Tissue
-
1:100-1:400
靶点
功能
Cytokeratin 15 (CK15, K15, K1CO, keratin15, type I cytoskeletal 15) is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains, and are clustered in a region on chromosome 17q21.2. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
背景文献
1. Pal SK et al. The expression profiles of acidic epithelial keratins in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol 115:523-31 (2013).
2. Tai G et al. Cytokeratin 15 marks basal epithelia in developing ureters and is upregulated in a subset of urothelial cell carcinomas. PLoS One 8:e81167 (2013).
亚细胞定位
Intermediate filament, cytosol, extracellular exosome, nucleus.
别名
AI528832 antibody
CK 15 antibody
CK-15 antibody
CK15 antibody
Cytokeratin-15 antibody
Cytokeratin15 antibody
K15 antibody
K1C15_HUMAN antibody
K1CO antibody
Ka15 antibody
展开图片
-
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720122F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/200 dilution at +4℃ overnight, washed with PBS.
Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720122F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/200 dilution at +4℃ overnight, washed with PBS.
Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720122F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720122F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"