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Goat anti-Mouse IgG-HRP antibody

羊多抗 | 货号: HA1006


规格:
价格:RMB   200
  • 概述

  • 产品描述 Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
  • 产品名称 Goat anti-Mouse IgG-HRP antibody
  • 种属反应性 Mouse
  • 验证应用 WB, ELISA, IHC
  • 抗体类型 羊多抗
  • 免疫原 Mouse IgG (H+L).
  • 偶联 HRP-conjugated
  • 性能

  • 形态 Liquid
  • 浓度 1 mg/ml.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol.
  • 亚型 IgG
  • 纯化方式 Protein affinity purified.
  • 应用

    WB: 1:5000- 1:2000
    ELISA: 1:5,000-1:10000
    IHC: 1:50-1:100

  • 背景文献

  • 暂无
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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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