• 概述

• 产品描述 Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
• 产品名称 Anti-beta Tubulin Recombinant Rabbit Monoclonal Antibody [SR25-04]
• 分子量 Predicted band size: 50 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 WB,IF-Cell,IHC-P,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human beta Tubulin aa 308-357 / 444.
• 偶联 Non-conjugated
• RRID AB_2938592

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm
• 数据链接 SwissProt: P07437 Human
  SwissProt: P99024 Mouse
  SwissProt: P69897 Rat
  
• 其它名称
  • Beta 4 tubulin antibody
  • Beta 5 tubulin antibody
  • BetaTubulin antibody
  • TBB5_HUMAN antibody
  • TUBB antibody
  • TUBB2 antibody
  • TUBB2A antibody
  • TUBB5 antibody
  • tubulin beta 2A antibody
  • Tubulin beta chain antibody
  • Tubulin beta-5 chain antibody
  more

• 应用

  WB: 1:10,000-1:20,000
  IF-Cell: 1:50-1:200
  IHC-P: 1:100-1:400
  FC: 1:500-1:1,000

• 

  Fig1: Western blot analysis of beta Tubulin on different lysates with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/10,000 dilution.
  
  Lane 1: HeLa cell lysate (15 µg/Lane)
  Lane 2: 293T cell lysate (15 µg/Lane)
  Lane 3: MCF7 cell lysate (15 µg/Lane)
  Lane 4: SH-SY5Y cell lysate (15 µg/Lane)
  Lane 5: U-2 OS cell lysate (15 µg/Lane)
  Lane 6: Jurkat cell lysate (15 µg/Lane)
  Lane 7: Neuro-2a cell lysate (15 µg/Lane)
  Lane 8: NIH/3T3 cell lysate (15 µg/Lane)
  Lane 9: PC-12 cell lysate (15 µg/Lane)
  Lane 10: Mouse brain tissue lysate (20 µg/Lane)
  Lane 11: Rat brain tissue lysate (20 µg/Lane)
  
  Predicted band size: 50 kDa
  Observed band size: 50 kDa
  
  Exposure time: 1 minute 20 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-4) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Immunocytochemistry analysis of SH-SY5Y cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  

  Fig3: Immunocytochemistry analysis of Hela cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  

  Fig4: Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  

  Fig5: ICC staining of beta Tubulin in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig6: ICC staining of beta Tubulin in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig7: ICC staining of beta Tubulin in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/100 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig9: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig10: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig11: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig12: Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin.
  
  Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-4, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• 背景文献

• 1. "Tumoral and tissue-specific expression of the major human beta-tubulin isotypes."Leandro-Garcia L.J., Leskela S., Landa I., Montero-Conde C., Lopez-Jimenez E., Leton R., .Cytoskeleton 67:214-223(2010).
  
  2. "Five mouse tubulin isotypes and their regulated expression during development."Lewis S.A., Lee M.G.-S., Cowan N.J.J. Cell Biol. 101:852-861(1985).

• Publishing research using Anti-beta Tubulin Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  36781842 Rab31 promotes metastasis and cisplatin resistance in stomach adenocarcinoma through Twist1-mediated EMT Author: Chen, K., Xu, J., Tong, Y. L., Yan, J. F., Pan, Y., Wang, W. J., Zheng, L., Zheng, X. X., Hu, C., Hu, X., Shen, X., & Chen, W. Published: 2023 Application: WB Species: Human Journal: Cell Death Dis IF: 8.469
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• PMID:
  36409110 Porcine Circovirus Type 2 Hijacks Host IPO5 to Sustain the Intracytoplasmic Stability of Its Capsid Protein Author: Lin, C., Hu, J., Dai, Y., Zhang, H., Xu, K., Dong, W., Yan, Y., Peng, X., Zhou, J., & Gu, J. Published: 2022 Journal: J Virol IF: 6.549
• PMID:
  36338340 Dynamin-Related Protein 1 Is Involved in Mitochondrial Damage, Defective Mitophagy, and NLRP3 Inflammasome Activation Induced by MSU Crystals Author: Jiang, H., Chen, F., Song, D., Zhou, X., Ren, L., & Zeng, M. Published: 2022 Application: WB Species: Mouse Journal: Oxid Med Cell Longev. IF: 7.310

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.